Abstract Glucagon-like peptide-2 receptor (GLP2R) is one of the key mediators of regulating body energy balance, which may have important effects on organ development and growth performance of sheep (Ovis aries). This study was conducted to investigate the association between GLP2R single nucleotide polymorphism and slaughter performance and weight of internal organs such as heart, liver, spleen, lung and kidney in Hu sheep. Firstly, the expression of GLP2R gene in different tissues was analyzed by qPCR. Furthermore, the polymorphism of GLP2R gene locus was detected by kompetitive allele specific PCR (KASP) typing technique in 1 351 male Hu lamb with complete pedigree, and the correlation analysis was conducted with the slaughter traits and weight of internal organs of lambs. The results showed that GLP2R gene was widely expressed in various tissues of Hu sheep, and the relative expression level was significantly higher in kidney and rumen tissues (P<0.05). There were 3 genotypes GG, GA and AA at g.34409249 G>A locus of GLP2R gene exon, which showed moderate polymorphism (0.25<PIC<0.50). This locus was not in the Hardy Weinberg equilibrium state in the population (P<0.05). The single nucleotide polymorphism of GLP2R gene was closely related to the heart, lung and kidney weight of Hu sheep, and the heart, lung and kidney weight of GG genotype group was significantly higher than that of AA genotype group (P<0.05). In conclusion, the mutation site of GLP2R gene exon in G.34409249 G>A can be used as a candidate molecular marker site for heart, lung and kidney organ development, which provides a theoretical basis for the breeding of Hu sheep.
HUANG Yong-Liang,WANG Wei-Min,WANG Xiao-Juan, et al. Polymorphism of GLP2R Gene and Its Association Analysis with Visceral Weight in Hu Sheep (Ovis aries)[J]. 农业生物技术学报, 2023, 31(2): 341-349.
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