毛竹<em>PheMOC1a/b</em>基因表达与互作蛋白分析

doi:10.3969/j.issn.1674-7968.2023.01.007

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Abstract Moso bamboo (Phyllostachys edulis) is an important economic bamboo species in China. The normal development of bamboo bud/shoot determines its yield. The MONOCULM1 (MOC1) gene plays an important role in the formation and development of axillary meristems and axillary buds. In this study, 2 MOC1 homologous genes, PheMOC1a (GenBank No. ON804247) and PheMOC1b (GenBank No. ON804248), were obtained from moso bamboo by RT-PCR. The sequence analysis showed that the ORFs of PheMOC1a and PheMOC1b were 1 530 and 1 293 bp, encoding 509 and 430 amino acid, respectively. Both polypeptide chains contain GRAS-superfamily conserved domains and belong to the GIBBERELLIN ACID INSENSITIVE (GAI), REPRESSOR of GA1 (RGA) and SCARECROW (SCR) (GRAS) gene family. Phylogenetic tree showed that PheMOC1a/b was closely related to MOC1 in rice (Oryza sativa). qRT-PCR results showed that the PheMOC1a and PheMOC1b showed obvious tissue-specific expression. PheMOC1a and PheMOC1b showed opposite expression characteristics in the bamboo bud/shoot at different developmental stages. With the development of bamboo bud/shoot, the expression of PheMOC1a decreased, while the expression of PheMOC1b increased. The trend of PheMOC1a and PheMOC1b expression was opposite in the branches at different developmental stages. The results of yeast two-hybrid and bimolecular fluorescence complementation showed that PheMOC1a and PheMOC1b not only interacted with themselves, but also interacted with each other, and interacted with 2 members of the indeterminate domain (IDD) family. These interacting proteins played biological roles in the nucleus. This study provides a reference for further analysis of the biological functions of PheMOC1a/b, and basic information for the molecular mechanism of bamboo bud/shoot development in moso bamboo.

Key words： Moso bamboo MONOCULM1 (MOC1) gene Bamboo shoot GRAS family Gene expression Protein interaction

Received: 25 April 2022

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S795.7

Corresponding Authors: *xqguo@zafu.edu.cn

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	Articles by authors
	SHAO Ming-Xia
	ZHOU Min-Shu
	CHEN Jiao-Yu
	LIU Li
	GUO Xiao-Qin

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SHAO Ming-Xia,ZHOU Min-Shu,CHEN Jiao-Yu, et al. PheMOC1a/b Gene Expression and Interaction Protein Analysis in Moso Bamboo (Phyllostachys edulis)[J]. 农业生物技术学报, 2023, 31(1): 73-86.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.01.007 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I1/73

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