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| Study on Plasma Metabolomics of Mongolian Horses (Equus caballus) and Hybrid Horses Before and After Endurance Exercise |
| WEI Rui-Yuan1, Yiboletu1, HAN Hai-Ge1, BAI Dong-Yi1, ZHAO Yi-Ping1, WANG Xi-Sheng1, REN Xiu-Juan1, MONGKE Togtokh1, MANG Lai1,*, LI Yu-Min2 |
1 College of Animal Science, Inner Mongolia Agricultural University/Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction/Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs/Inner Mongolia Agricultural University Equine Research Center, Hohhot 010018, China;
2 Beijing Military Region Hongshan Horse Field, Chifeng 025377, China |
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Abstract Exercise metabolomics is a systems biology study that evaluates the overall metabolic response of the body to exercise training stimulation. Recent years, mainly researches have focused on the sports science in human (Homo sapiens), and there are few reports on the study of Chinese horse (Equus caballus) sports metabolomics. In this study, 1H nuclear magnetic resonance (1H-NMR) was used to analyze the characteristics of plasma metabolomics of 6 Mongolian horses and 6 hybrid horses before and after 30 km endurance exercise, and metabolic patterns and differential metabolic markers between groups were identified by partial least square discriminant analysis (PLS-DA) package of R language and one-dimensional ANOVA test of SPSS 20.0 software. There were distinct metabolic models between 2 horse groups before and after exercise. The results showed that there were significant differences in metabolic patterns between Mongolian horses and hybrid horses before and after exercise. Ten differential metabolites were identified before and after Mongolian horse exercise in whcih the concentration of glucose, ethanol, glycine, glutamine, lysine, proline, valine, and τ-methylhistidine decreased and the concentration of methanol and creatine increased. At the same time, 15 differential metabolites were identified before and after hybrid horse exercise in which the concentration of glucose, hippurate, acetate, glutamine, valine, methanol, betaine, τ-methylhistidine decreased and the concentration of lactate, glycerol, acetone, 3-hydroxybutyrate, 3-Hydroxyisobutyrate, acetoacetate and creatinine increased. The comprehensive analysis of metabolite changes showed that the energy supply mode of Mongolian horses was mainly aerobic metabolism, while the energy supply mode of hybrid horses was mainly anaerobic metabolism of phosphate source and glycolysis, as well as fatty acid ketone metabolism. Therefore, Mongolian horses had better aerobic endurance quality and explosive potential than hybrid horse, so it is feasible to breed endurance racehorses based on Mongolian horse; and glutamine and τ-methylhistidine could be developed and applied as a monitoring indicators for horse endurance training. The results of this study provides basic data for endurance training and breeding of Mongolian horses in China.
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Received: 10 December 2021
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Corresponding Authors:
* dmanglai@163.com
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