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| Identification and Expression Analysis of AQP Gene Family Members in Peach (Prunus persica) |
| XU Yao-Guang, ZHANG Zheng-Quan, YU Yang, GUAN Jian-Tao, XIE Hua* |
| The Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology/Biotechnology Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China |
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Abstract Aquaporins (AQPs) are highly conserved membrane proteins that facilitate the passive movement of water and other small hydrophilic molecules across biological membranes, playing essential roles in the physiological processes of seed germination, flowering, and fruit development, etc. In this study, 28 APQ gene family members (PpAQP1~PpAQP28) were identified from the peach (Prunus persica) genome, which could be categorized into 5 subfamilies, including plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin-26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs) and uncategorized X intrinsic proteins (XIPs) based on their phylogenetic relationships. Analyses of the chromosomal assignment, gene structures and conserved motifs of these PpAQP members were further performed. Combining with the transcriptome and qPCR detection, tissue-specific expression profiles of these PpAQP genes and their expression patterns at various fruit development stages were analyzed. These results demonstrate the comprehensive expression differentiations of PpAQPs among and within the sub-families, 8 PpAQPs were highly expressed in fruits with their expression high peaks in the early stage of fruit development, suggested their potential roles in the first rapid growth period of fruit development. This research offers a reference for subsequent functional studies of AQPs in fruit development.
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Received: 24 August 2021
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Corresponding Authors:
*xiehua@baafs.net.cn
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[1] 邓晓旭, 陈黄曌, 王琦 . 2017. 植物水通道蛋白调控及其基因功能研究进展[J]. 中国农业信息 , 10: 37-38.
(Deng X X, Chen H Z, Wang Q.2017. Research progress on regu‐ lation and gene function of aquaporin in plants[J]. Agri‐ cultural Information, 10: 37-38.)
[2] Afzal Z, Howton T C, Sun Y, et al.2016. The roles of aquapo‐ rins in plant stress responses[J]. The International Jour‐ nal of Developmental Biology, 4(1): 9-30.
[3] Ahamed A, Murai-Hatano M, Ishikawa-Sakurai J, et al. 2012. 1)Cold stress-induced acclimation in rice is mediated by root specific aquaporins[J]. Plant and Cell Physiology, 53(8): 1445-1456.
4 2)Anderberg H I, Kjellbom P, Johanson U.2012. Annotation of Selaginella moellendorffii major intrinsic proteins and the evolution of the protein family in terrestrial plants[J]. Frontiers in Plant Science, 3(33): 33-46.
[4] Aritua V, Achor D, Gmitter F G, et al.2013. Transcriptional and microscopic analyses of citrus stem and root re‐ sponses to Candidatus Liberibacter asiaticus infection[J]. PLOS ONE, 8(9): e73742.
[5] Azad A K, Ahmed J, Alum M A, et al.2018. Prediction of ar‐ senic and antimony transporter major intrinsic proteins from the genomes of crop plants[J]. International Jour0 Bezerra-Neto J P, de Araujo F C, Ferreira-Neto J, et al. 2019. Plant aquaporins: Diversity, evolution and biotechnological applications[J]. Current Protein & Peptide Science, 20(4): 368-395.
[6] Chaumont F, Tyerman S D.2014. Aquaporins: Highly regulat‐ ed channels controlling plant water relations[J]. Plant Physiology, 164(4): 1600-1618.
[7] Chen G P, Wilson I D, Kim S H, et al.2001. Inhibiting expression of a tomato ripening-associated membrane protein increases organic acids and reduces sugar levels of fruit[J]. Planta, 212(5-6): 799-807.
[8] Coombe B G.1976. The development of fleshy fruits[J]. Annual Review of Plant Physiology, 27(1): 207-228. Danielson J A, Johanson U. 2008. Unexpected complexity of the aquaporin gene family in the moss Physcomitrellapatens[J]. BMC Plant Biology, 8: 45.
[9] Deshmukh R, Belanger R.2016. Molecular evolution of aqua‐ porins and silicon influx in plants[J]. Functional Ecolo‐ gy, 8(30): 1277-1285.
[10] Eriksson U K, Fischer G, Friemann R, et al.2013. Subangstrom resolution X-ray structure details aquaporin-water interactions[J]. Science, 340(6138): 1346-1349.
[11] Etienne A, Genard M, Lobit P, et al.2013. What controls fleshy fruit acidity? A review of malate and citrate accu‐ mulation in fruit cells[J]. Journal of Experimental Bota‐ ny, 64(6): 1451-1469.
[12] Fouquet R, Leon C, Ollat N, et al.2008. Identification of grapevine aquaporins and expression analysis in devel‐ oping berries[J]. Plant Cell Reports, 27(9): 1541-1550.
[13] Guan J, Xu Y, Yu Y, et al.2021. Genome structure variation analyses of peach reveal population dynamics and a 1.67Mb causal inversion for fruit shape[J]. Genome Biology, 22(1): 13.
[14] Hu W, Hou X, Huang C, et al.2015. Genome-wide identifica‐ tion and expression analyses of aquaporin gene family during development and abiotic stress in banana[J]. In‐ ternational Journal of Molecular Sciences, 16(8): 19728-19751.
[15] Johanson U, Karlsson M, Johansson I, et al.2001. The com‐ plete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomencla‐ ture for major intrinsic proteins in plants[J]. Plant Physiology, 126(4): 1358-1369.
[16] Kapilan R, Vaziri M, Zwiazek J J.2018. Regulation of aqua‐ porins in plants under stress[J]. Biological Research, 51(1): 4.
[17] Katsuhara M, Akiyama Y, Koshio K, et al.2002. Functional analysis of water channels in barley roots[J]. Plant and Cell Physiology, 43(8): 885-893.
[18] Katsuhara M, Koshio K, Shibasaka M, et al.2003. Over-ex‐ pression of a barley aquaporin increased the shoot/root ratio and raised salt sensitivity in transgenic rice plants[J]. Plant and Cell Physiology, 44(12): 1378-1383.
[19] Lopez-Zaplana A, Nicolas-Espinosa J, Carvajal M, et al.2020. Genome wide analysis of the aquaporin genes in melon (Cucumis melo L.)[J]. Scientific Reports, 10(1):22240.
[20] Martins C P, Pedrosa A M, Du D, et al.2015. Genome-wide characterization and expression analysis of major intrin‐ sic proteins during abiotic and biotic stresses in sweet orange (Citrus sinensis L. Osb.)[J]. PLOS ONE, 10(9): e138786.
[21] Maurel C.2007. Plant aquaporins: novel functions and regulation properties[J]. FEBS Letters, 581(12): 2227-2236. Maurel C, Boursiac Y, Luu D T, et al. 2015. Aquaporins in plants[J]. Physiological Reviews, 95(4): 1321-1358.
[22] Mut P, Bustamante C, Martinez G, et al.2008. A fruit-specific plasma membrane aquaporin subtype PIP1; 1 is regulat‐ ed during strawberry (Fragaria x ananassa) fruit ripening[J]. Physiologia Plantagrum, 132(4): 538-551.
[23] Noronha H, Agasse A, Martins A P, et al.2014. The grape aquaporin VvSIP1 transports water across the ER mem‐ brane[J]. Journal of Experimental Botany, 65(4): 981-993.
[24] Park W, Scheffler B E, Bauer P J, et al.2010. Identification of the family of aquaporin genes and their expression in up‐ land cotton (Gossypium hirsutum L.)[J]. BMC Plant Biol‐ ogy, 10: 142.
[25] Reuscher S, Akiyama M, Mori C, et al.2013. Genome-wide identification and expression analysis of aquaporins in tomato[J]. PLOS ONE, 8(11): e79052.
[26] Rizhsky L, Liang H, Shuman J, et al.2004. When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress[J]. Plant Physiology, 134(4): 1683-1696.
[27] Sakurai J, Ishikawa F, Yamaguchi T, et al.2005. Identification of 33 rice aquaporin genes and analysis of their expression and function[J]. Plant Cell Physiology, 46(9): 1568-1577.
[28] Shivaraj S M, Deshmukh R K, Rai R, et al.2017. Genomewide identification, characterization, and expression pro‐ file of aquaporin gene family in flax (Linum usitatissi- mum)[J]. Scientific Reports, 7: 46137.
[29] Tong Z, Gao Z, Wang F, et al.2009. Selection of reliable reference genes for gene expression studies in peach using realtime PCR[J]. BMC Molecular Biology, 10(1): 71.
[30] Vandeleur R K, Mayo G, Shelden M C, et al.2009. The role of plasma membrane intrinsic protein aquaporins in wa‐ ter transport through roots: diurnal and drought stress re‐ sponses reveal different strategies between isohydric and anisohydric cultivars of grapevine[J]. Plant Physiol‐ ogy, 149(1): 445-460.
[31] Verde I, Abbott A G, Scalabrin S, et al.2013. The high quality draft genome of peach (Prunus persica) identifies uniquepatterns of genetic diversity, domestication and genome evolution[J]. Nature Genetics, 45(5): 487-494.
[32] Wada H, Shackel K A, Matthews M A.2008. Fruit ripening in Vitis vinifera: Apoplastic solute accumulation accounts for pre-veraison turgor loss in berries[J]. Planta, 227(6):1351-1361.
[33] Wong D, Zhang L, Merlin I, et al.2018. Structure and tran‐ scriptional regulation of the major intrinsic protein gene family in grapevine[J]. BMC Genomics, 19(1): 248.
[34] Yamaguchi-Shinozaki K, Koizumi M, Urao S, et al.1992. Molecular cloning and characterization of 9 cDNAs for genes that are responsive to desiccation in Arabidopsis thaliana: Sequence analysis of one cDNA clone that en‐ codes a putative transmembrane channel protein[J]. Plant and Cell Physiology, 3(33): 217-224.
[35] Yuan D, Li W, Hua Y, et al.2017. Genome-wide identification and characterization of the aquaporin gene family and transcriptional responses to boron deficiency in Brassica napus[J]. Frontiers in Plant Science, 8: 1336.
[36] Zhang D Y, Ali Z, Wang C B, et al.2013. Genome wide sequence characterization and expression analysis of ma-jor intrinsic proteins in soybean (Glycine max L.)[J]. PLOS ONE, 8(2): e56312. |
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