大豆根际踝节真菌WR1-4的溶磷特性研究

doi:10.3969/j.issn.1674-7968.2022.01.011

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Abstract Phosphorus solibilizating fungus can convert the potentially ineffective phosphorus into available phosphorus in the soil, which is an effective way to alleviate the phenomenon of soil phosphorus deficiency and increase the utilization rate of phosphorus fertilizer. The strain WR1-4 (Talaromyces sp.) was screened from the rhizosphere soil of soybean (Glycine max) in preliminary work. In order to clarify its phosphorus solubility and acid production, the phosphate solubilizing ability of strain WR1-4 was measured by plating method and broth method in present study; the molybdenum antimony anti colorimetric method was used to detect the phosphorus solubilizing characteristics of strain WR1-4 under different conditions of insoluble phosphorus, carbon and nitrogen sources; HPLC was used to detect the organic acids produced by WR1-4. The results showed that WR1-4 had different ability to dissolve 5 insoluble phosphates (calcium phosphate, zinc phosphate, aluminum phosphate, phosphate rock powder, iron phosphate), and the effective phosphorus increments were 693.07, 613.47, 260.14, 137.84 and 386.33 mg/L, respectively. Strain WR1-4 used 6 carbon sources in the order of glucose, sucrose, maltose, starch, lactose and cellulose. As to different nitrogen sources, strain WR1-4 had strongest phosphate solubilizing capacity under the condition of ammonium sulfate, and least phosphate solubilizing ability under the condition of potassium nitrate. Strain WR1-4 could produce 8 kinds of organic acids, including oxalic acid, lactic acid, citric acid, malic acid, acetic acid, fumaric acid, tartaric acid, and maleic acid. According to Pearson analysis, the correlation between the increase of available phosphorus and organic acid was positively correlated. At the same time, while strain WR1-4 was in the stage of phosphate solubilization, the pH of the environment decreased. Above results indicate that strain WR1-4 had a strong ability to dissolve phosphorus and a wide range of growth and metabolism conditions, which makes it has potential application prospects in microbial fertilizer development and agricultural production.

Key words： Phosphate solubilizing fungus Carbon source Nitrogen source Organic acid

Received: 06 April 2021

ZTFLH:	S154.3
	S565.1

Corresponding Authors: *wangjihua333@hotmail.com

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	REN Qing-Xin
	WANG Ji-Hua
	ZENG Qi
	ZHANG Bi-Xian
	LIU Xiu-Lin

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REN Qing-Xin,WANG Ji-Hua,ZENG Qi, et al. Study on Phosphorus Solubilization Characteristics of Talaromyces sp. WR1-4 in Soybean (Glycine max) Rhizosphere[J]. 农业生物技术学报, 2022, 30(1): 114-124.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2022.01.011 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2022/V30/I1/114

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