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| Identification and Expression Analysis of Transcription Factor MYB Family Genes in Botrytis cinerea |
| ZHU Qian-Jie1,2,*, ZHU Zi-Ping3,*, YU Xin-Yue2, ZHANG Qiang2, CAO Hong-Zhe2, XING Ji-Hong1,2, ZHANG Kang1,2,**, DONG Jin-Gao1,2,** |
1 State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, China;
2 Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding 071000, China;
3 Langfang Academy of Agriculture and Forestry Science, Langfang 065000, China |
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Abstract MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factors are widely present in plant pathogenic fungi, and play a variety of roles in the growth, development and stress response of fungi. In order to understand the function of the MYB transcription factor family of Botrytis cinerea, this study used bioinformatics methods to conduct phylogenetic analysis, conserved domain analysis, and analysis of the MYB transcription factor family in B. cinerea. Analysis of the expression regularity during the infection period; and using qPCR technology to detect the expression of the MYB family genes of B. cinerea after NaCl and H2O2 treatment. The results showed that there were 22 MYB transcription factor family genes in Botrytis cinerea, and phylogenetic analysis divided them into 6 subfamilies, namely MYB, MYB-Hox, Zn-ZZ-MYB, DEXDc-HELICc-MYB, HAS- MYB and BAH-PND-MYB-Zn-GATA; MYB family genes showed different expression patterns at different developmental stages and infection stages of conidia, and different expression patterns after NaCl and H2O2 treatments, indicating that the genes of MYB family of B. cinerea might play an important role in the growth and development of the pathogen, the process of infection and responding to stress. The present study provides basic material for studying the gene function of the MYB transcription factor family and the control of B. cinerea.
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Received: 30 March 2021
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Corresponding Authors:
**zk7588@163.com; dongjingao@126.com
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| About author:: * These authors contributed equally to this work |
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