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| Study on Differential Tissue Expression of MEF2C Splice Variants in Xingyi Duck (Anas platyrhynchos) |
| YE Tao1,2,3, YANG Hua-Ting1,2,3, LI Hui1,2,3,*, LUO Wei1,2,3, XIAO Tao1,2,3, LI Xing-Cai1,2,3, WU Gu-Rong4 |
1 Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China;
2 Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, China;
3 College of Animal Science, Guizhou University, Guiyang 550025, China;
4 Agriculture and Rural Affairs Bureau of Xingyi City, Qiandongnan Prefecture 562400, China |
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Abstract At present, the expression of splice variants of myocyte enhancer factor 2C gene (MEF2C) in ducks (Anas platyrhynchos) has not been deeply studied. In order to understand the expression difference of MEF2C variants in tissues, RT-PCR was used to clone the full-length CDS of MEF2C gene in Xingyi duck. The results showed that 2 splice variants of MEF2C-a (GenBank No. MZ497418) and MEF2C-b (GenBank No. MZ497419) were successfully cloned, and the encoded amino acid sequences shared more than 95% homology with pigeon (Columba livia), quail (Coturnix japonica) and chicken (Gallus gallus), which indicated that MEF2C gene was highly conserved in the evolution process. The results of qPCR showed that MEF2C-a and MEF2C-b were expressed in both male and female ducks, and highly expressed in brain, myocardium, leg muscle and chest muscle. The expression patterns of the 2 splice variants in female and male ducks were different. The expression level of MEF2C-a in most tissues of male ducks was higher than that of MEF2C-b, the opposite in female ducks. The expression of 2 splice variants in leg muscles and chest muscles did not increase with increasing age. The present results provide basic materials for further study on the mechanism of MEF2C splice variants in duck muscle development.
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Received: 06 January 2021
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Corresponding Authors:
* ellenlihui@sina.cn
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