矮小体型芦花鸡<em>GHR</em>基因突变类型分析及其与正常体型芦花鸡发育差异研究

doi:10.3969/j.issn.1674-7968.2021.10.012

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Abstract Due to its small size and saving breeding space, dwarf chickens (Gallus domesticus) are often used in the production of chicken supporting lines. The purpose of this study was to clarify the type of growth hormone receptor (GHR) gene mutations in dwarf size Plymouth Rock chicken and to explore the differences in growth and development between the normal size Plymouth Rock chicken and the dwarf size Plymouth Rock chicken. Primers were designed for the four common mutation sites of GHR gene in dwarf size Plymouth Rock chickens. PCR amplification was performed using gDNA of dwarf size Plymouth Rock chickens as templates to analyze the genetic variation types. At the same time, the developmental characteristics of body weight and tibia length of normal and short size Plymouth Rock chickens were compared and analyzed. Three mathematical models of Logistic, Gompertz and Von Bertalanffy were used to analyze the optimal model and fitting parameters. The results showed that compared with the normal size Plymouth Rock chicken, the 1 781 bp deletion of the GHR gene in the dwarf sized Plymouth Rock chicken was the reason for its dwarfing. Normal and dwarf size Plymouth Rock chickens have obvious differences in development during 1 to 13 weeks of age, especially in body weight and tibia length. Logistic was the best fit model for body weight of normal and dwarf size Plymouth Rock chickens (R²>0.990). In terms of tibia length, among male Plymouth Rock chickens, the best fit model for normal sized Plymouth Rock chickens was Logistic, and the best fit model for dwarf sized Plymouth Rock chicken was Von Bertalanffy. Among female Plymouth Rock chickens, the best fit model for normal and dwarf sized Plymouth Rock chickens was Von Bertalanffy. This study provides a reference basis for fine breeding management of Plymouth Rock chickens and molecular marker-assisted breeding of the dwarf size Plymouth Rock chickens.

Key words： Dwarf size Plymouth Rock chicken Normal size Plymouth Rock chicken Growth hormone receptor (GHR) gene Mutation type Strain difference

Received: 01 February 2021

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Corresponding Authors: * zhangli761101@163.com

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	ZHENG Jia-Hui
	LENG Qi-Ying
	ZHANG Wei-Lu
	Ali Hassan NAWAZ
	DU Bing-Wang
	ZHANG Li

Cite this article:

ZHENG Jia-Hui,LENG Qi-Ying,ZHANG Wei-Lu, et al. Analysis of GHR Mutation Types in Dwarf Size Plymouth Rock Chicken (Gallus domesticus) and Study on Its Developmental Difference with the Normal Size One[J]. 农业生物技术学报, 2021, 29(10): 1978-1989.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.10.012 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I10/1978

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