假禾谷镰孢菌弱毒菌株FC136-2A的GFP标记转化子构建

doi:10.3969/j.issn.1674-7968.2021.07.020

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Abstract Wheat crown rot is an important soil-borne disease occurring widely in most wheat (Triticum aestivum) growing areas in the world. Fusarium pseudograminearum is the dominant causal agent of wheat crown rot. In F. pseudograminearum strain FC136-2A, mycovirus Fusarium pseudograminearum megabirnavirus 1 (FpgMBV1) was found to cause the hypovirulence of FC136-2A on wheat. To explore the mechanism of hypovirulence caused by FpgMBV1, a pCAM-GFP-hyg construct was transformed into F. pseudograminearum strain FC136-2A by Agrobacterium tumefaciens-mediated genetic transformation technology. Molecular identification, fluorescent observation, biological characterization and pathogenicity test of the transformants were also conducted. Results showed that the GFP-labeled transformant FC136-2A-GFP had no biological differences compared to FC136-2A in growth rate and sporulation capacity. Besides, FpgMBV1 was still in the transformant FC136-2A-GFP and the pathogenicity of FC136-2A-GFP was low on wheat. The present study obtained GFP-labeled transformant FC136-2A-GFP of F. pseudograminearum, which could be used to observe the infection and pathogenic process of hypovirulence strain FC136-2A on wheat, and provide basic information for further study on the mechanism of hypovirulence effect of mycovirus FpgMBV1 on F. pseudograminearum.

Key words： Wheat crown rot Fusarium pseudograminearum Mycovirus Fusarium pseudograminearum megabirnavirus 1 (FpgMBV1) Hypovirulence.

Received: 04 November 2020

ZTFLH:

S432.2+3

Corresponding Authors: *zhangxiaoting921@163.com;honglianli@sina.com

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	Articles by authors
	WANG Zhi-Fang
	LI Ke
	LIU Dong-Wei
	XIE Yuan
	DAI Jun-Li
	WANG Ke
	GAO Fei
	ZHANG Xiao-Ting
	LI Hong-Lian

Cite this article:

WANG Zhi-Fang,LI Ke,LIU Dong-Wei, et al. Construction of GFP-labeled Transformant of Hypovirulent Strain FC136-2A in Fusarium pseudograminearum[J]. 农业生物技术学报, 2021, 29(7): 1435-1442.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.07.020 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I7/1435

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