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| Cloning and Expression Analysis of 6-gingerol Biosynthesis Related Genes from Zingiber officinale |
| TANG Jian-Min1, QI Li-Wang2, LANG Mei-Rong1, ZHANG Wen-Lin1, LAN Jian-Bin1, LI Hong-Lei1, LI Zhe-Xin1,* |
1 Chongqing Key Laboratory of Economic Plant Biotechnology/Chongqing Special Plant Industry Collaborative InnovationCenter/College of Landscape Architecture and Life Science/Institute of Special Plants, Chongqing University of Arts and Sciences, Chongqing 402160, China;
2 State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China |
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Abstract Ginger (Zingiber officinale) has high economic value as medicinal and food resources. 6-gingerol is high important bioactive component in the pharmacological action of ginger. In the present study, a local ginger cultivar of Chongqing was used as the material. Liquid chromatograph-mass spectrometer (LC-MS) technology was used to detect the main components of pungent principle. Four cDNA sequences of 6-gingerol biosynthetic relative enzyme genes including phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate:CoA ligase (4CL) and caffeoyl-CoA-o-methyltransferase (CCoAOMT/CCOMT) (GenBank No. MN887496, MN887498, MN887497 and MN887499) were cloned and qRT-PCR was used to detect the expression of the genes in ginger rhizome at different developmental stages. Correlation analysis was performed between the expression of each gene and the 6-gingerol increase.The results showed that 6-gingerol accumulated very quickly in the 1-month ginger material after sowing, and increased slightly in the subsequent stages. PAL, C4H, 4CL and CCoAOMT genes were all highly expressed in 1~2 months ginger after sowing, suggesting a correlation between each gene and 6-gingerol synthesis. Correlation analysis showed that the expression of CCoAOMT rather than the others was significantly positively correlated with 6-gingerol synthesis (P<0.05). These results indicated that the biosynthesis of 6-gingerol existed stage specificity, and there was a positive regulatory relationship between CCoAOMT gene and 6-gingerol biosynthesis. The results of this study will lay the foundation for the further study of the function of 6-gingerol synthesis-related enzyme genes in Z. officinale.
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Received: 16 May 2020
Published: 01 March 2021
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Corresponding Authors:
*lizhexin_8903@163.com
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