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| Expression Characteristics of TFEB Gene and Its Relationship with the Number of Lysosomes in Adipocytes in Broilers (Gallus gallus) |
| GAO Zhuo-Ran, DING Ran, CHEN Yao-Feng, ZHAI Xiang-Yun, LI Hui, DU Zhi-Qiang* |
| Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture / Key Laboratory of Animal Genetics, Breeding and Reproduction, Ordinary Institution of Higher Education Department of Heilongjiang Province / College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China |
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Abstract On the lysosome surface mammalian target of rapamycin (mTOR) can phosphorylate transcription factor EB (TFEB) to cause nuclear translocation and thus plays a biological role in lysosomal biosynthesis, autophagy and lipid metabolism. In this study, The Northeast Agricultural University High and Low Fat (NEAUHLF) broiler (Gallus gallus) lines were used to detect the expression of TFEB in abdominal fat tissue and adipocytes by qRT-PCR. In addition, TFEB protein was localized in broiler adipocytes by immunofluorescence, and the changes of lysosome number during the differentiation of broiler adipocytes were analyzed. The results showed that the expression of TFEB in abdominal fat of lean line was higher than that of fat line at 1, 4 and 7 weeks of age, and the expression of TFEB in abdominal fat of 2 lines was significantly different at 4 weeks of age. During the differentiation the expression of TFEB in primary preadipocytes of 2 lines showed an opposite trend. To be precise, the expression of TFEB decreased during the differentiation of fat line broilers and increased in that of lean lines. At the same time, the expression of TFEB decreased during the differentiation of primary preadipocytes of arbor acres (AA) broilers, which was significantly different between 72 h and 0 h. Moreover, TFEB protein was detected in the cytoplasm and nucleus of adipocytes of broilers and the number of lysosomes decreased during adipocyte differentiation. The results would lay the foundation for further investigating the molecular mechanisms of how TFEB regulates lysosome biogenesis and affects the fat metabolism in broilers.
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Received: 29 May 2019
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Corresponding Authors:
*zhqdu@neau.edu.cn
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