玉米大斑病菌转录因子StMR1的结构特征及其表达模式分析

doi:10.3969/j.issn.1674-7968.2019.10.002

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Abstract As an important pathogenic factor, melanin directly affects the infection ability of plant pathogenic fungi, and the transcription factor MR (melanin regulation factor) plays an important role in melanin biosynthesis. In this study, the transcription factor gene Stmr1 (GenBank No. NW007359937) in Setosphaeria turcica was identified and cloned through genome BLAST. The gene was 3 282 bp in length and consisted of 3 introns and 4 exons, encoding 1 021 amino acids. The homology analysis of amino acid sequence indicated that the encoded protein StMR1 had the highest similarity to the MR transcription factors of Alternaria brassicicola and Cochliobolus heterostrophus. It contained 2 Cys2His2 zinc finger motifs and 1 Zn(Ⅱ)2Cys6 zinc cluster protein. Self-activation assay showed that StMR1 had high transcriptional activation activity and was located in the nucleus by fluorescence localization. Detected by qRT-PCR, the expression of Stmr1 gene was significantly different in the 5 stages from germination of conidia to penetration, and the expression was significantly up-regulated in the invasive nail stage, so it was speculated that it was involved in infection and expansion of the pathogen. The results of this study provide a theoretical basis for further exploration of the mechanism of regulation of melanin synthesis and StMR1 transcription factors in germ infection.

Key words： Setosphaeria turcica Transcription factor Melanin regulation factor Gene structure Expression pattern

Received: 21 June 2019

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Corresponding Authors: caoyan208@126.com; dongjingao@126.com

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	Articles by authors
	JIA Hui
	ZHANG Ze-Xue
	Liu Ning
	MENG Qing-Jiang
	CAO Zhi-Yan
	DONG Jin-Gao

Cite this article:

JIA Hui,ZHANG Ze-Xue,Liu Ning, et al. Structural Characteristics and Expression Pattern Analysis of Transcription Factor StMR1 in Setosphaeria turcica[J]. 农业生物技术学报, 2019, 27(10): 1720-1728.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2019.10.002 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2019/V27/I10/1720

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