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| Cloning and Expression of ZmXTH23 in Maize (Zea mays) and Its Response to Salt and Drought Stress |
| CHEN Dong-Bin1, 3, WANG Qian-Qian2, 3, SUN Zhi-Yi2, 3, YANG Xiao-Ying2, 3, FU Jing-Xiao2, 3, GUO Xin-Mei2, 3, *, SONG Xi-Yun2, 3, * |
1 College of Life Science, Qingdao Agricultural University, Qingdao 266109, China;
2 College of Agronomy, Qingdao Agricultural University, Qingdao 266109, China;
3 Key Laboratory of Major Crop Germplasm Innovation and Application in Qingdao, Qingdao 266109, China |
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Abstract Xyloglucan transglycosidase/hydrolase (XTH) is a key enzyme in the process of plant cell wall remodeling, and participates in the regulation of plant growth and development. To elucidate and explore the function of XTH in the process of stress response in maize (Zea mays), ZmXTH23 (GenBank No. LOC100191584) in XTH family was cloned from maize inbred line 'Chang7-2' and its biological function was studied. Bioinformatics analysis showed that ZmXTH23 contained a complete open reading frame of 897 bp encoded 298 amino acids and was a member of LamG superfamily. Besides, it performed xyloglucan endotransglycosidase (XET) activity and its amino acid sequence had the closest affinity with that of PmXTH25 (GenBank No. RLM56175.1) in Panicum miliaceum (similarity reached 90%). The expression pattern of ZmXTH23 was analyzed by qRT-PCR, and the results showed that the expression of ZmXTH23 was tissue-specific, it had the highest expression level in young stem (P＜0.01), and was induced by abscisic acid (ABA), NaCl and PEG6000. Prokaryotic expression analysis by SDS-PAGE and Western blot confirmed that ZmXTH23 protein could be expressed in Escherichia coli BL21 transferred with pET28a-ZmXTH23 recombinant plasmid. Besides, the growth of host bacteria pET28a-ZmXTH23 under different concentrations of salt and mannitol stress was better than that of host bacteria pET28a, which indicated that the ZmXTH23 protein enhanced the salt tolerance and drought resistance of pET28a-ZmXTH23 recombinant host strain. In conclusion, ZmXTH23 played an important role in response to abiotic stress and the results in this study provides theoretical basis for the creation of new maize germplasm resistant to stress.
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Received: 29 January 2019
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Corresponding Authors:
xmguo2009@126.com; songxy@qau.edu.cn
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