VRTN及NR6A1基因多态性与苏尼特羊胸椎数变异的相关性及组织表达分析

doi:10.3969/j.issn.1674-7968.2019.05.010

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Abstract The number of thoracic vertebrae is an important economic trait in production, but few candidate genes have been identified in sheep (Ovis aries). The aim of this study is to explore the association between polymorphism of VRTN (vertnin) and NR6A1 (nuclear receptor subfamily 6 group A member 1) genes and thoracic vertebral number, and tissue expression levels of 2 genes were also detected. The association analysis of VRTN and NR6A1 polymorphisms with thoracic vertebral number were performed by PCR, and expression levels of VRTN and NR6A1 genes in Sunite sheep with different thoracic vertebral numbers (14 thoracic vertebral number, T14; 13 thoracic vertebral number, T13) were tested by qRT-PCR. The results showed that there were no significant association between g.82533904C>T, g.11206763T>C and g.11210075T>C and the thoracic vertebral number in Sunite sheep (P＞0.05), while there was a highly significant association between g.82533661C>A and the thoracic vertebral number in Sunite sheep (P＜0.01). NR6A1 expressed in heart, liver, spleen, lung, kidney, muscle and fat of Sunite sheep. There were no significant difference of expression levels between sheep with T14 and T13 in heart, lung, kidney, muscle and fat (P＞0.05), while there were significant difference in spleen (P＜0.05) and extremely significant difference in liver (P＜0.01) of Sunite sheep with different thoracic vertebral number. The expression levels of VRTN were all higher in Sunite sheep with T14 than those of T13 in heart, liver, spleen, lung and kidney, the difference of muscle was significant (P＜0.05), the difference of the other tissues were not significant (P＞0.05). There was no uniform expression pattern of Sunite sheep with different thoracic vertebral number. In summary, g.82533661C>A had potential application value for the selection of the Sunite sheep population with more thoracic vertebral number. At the same time, there was no significant association between NR6A1 and the thoracic vertebral trait of the Sunite sheep, and VRTN may be one of the candidate genes affecting the thoracic vertebral numbers in Sunite sheep. Determining candidate genes for thoracic vertebrae number trait in sheep is conducive to the selection of multiple thoracic vertebrae sheep population, which can effectively improve the economic benefits of the sheep industry.

Key words： VRTN NR6A1 Thoracic vertebral number Polymorphism Tissue expression Sunite sheep

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	Articles by authors
	SUN Qing
	LIU Shu-Jun
	DI Ran
	HU Wen-Ping
	WANG Xiang-Yu
	MA Lin
	ZHANG Xiao-Sheng
	ZHANG Jin-Long
	LIU Qiu-Yue
	CHU Ming-Xing

Cite this article:

SUN Qing,LIU Shu-Jun,DI Ran, et al. Association Between Polymorphism of VRTN, NR6A1 Genes and Thoracic Vertebral Number Variation and Analysis of Their Tissue Expression in Sunite Sheep (Ovis aries)[J]. 农业生物技术学报, 2019, 27(5): 864-874.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2019.05.010 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2019/V27/I5/864

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