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| Screening and Analysis of Molecular Markers for Pod Rot Resistance in American Peanut (Arachis hypogaea) Germplasm Resources |
| LIU Yang-Jie1, *, HE Mei-Jing1, *, CUI Shun-Li1, YANG Xin-Lei1, MU Guo-Jun1, Charles Y CHEN2, LIU Li-Feng1, ** |
1 Hebei Agricultural University, Baoding 071001, China;
2 Auburn University, Auburn, AL 36849, USA |
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Abstract Peanut (Arachis hypogaea) pod rot, a worldwide fungal disease, has become one of the important diseases that directly affect the production and quality of peanut. Pod rot, as a kind of soil-borne disease, is difficult to be completely controlled with chemicals, cultivation methods or even biological techniques. Disease resistance breeding is an economical and effective way to control the pod rot. The screening and utilization of resistance related molecular markers will be an important means to improve the selection efficiency and speed up the breeding process. According to the results of the previous study, 12 pairs of SSR markers related to peanut disease resistance in references and 48 pairs of insertion-deletion (InDel) markers developed based on the result of stress resistance in this lab were selected to detect 77 American peanut germplasm resources, and the correlation between pod rot resistance and markers were analyzed. Pearson correlation analysis and multiple linear stepwise regression analysis were conducted based on the evaluation criteria of peanut pod rot resistance established in this laboratory. And the associated SSR markers of pod rot were validated with a following F2 segregation-population. The results revealed that there were abundant of genetic diversity and disease resistance in American germplasm resources, and inter-annual environmental factors had a significant impact on disease resistance of peanut germplasm. The results of SSR polymorphism showed that there were 103 polymorphic bands and the polymorphic rate of 20% to 100% with an average of 79.23% among 77 peanut germplasm. The results of correlation and multiple linear stepwise regression analysis between injury index and SSR markers revealed that four markers including 3A8, GM1760, PM163 and 14H6 were significantly associated with peanut pod rot resistance. These 4 markers were considered could be well used in further study on the development of molecular markers related to peanut pod rot resistance. The validation results in F2 population showed that SSR marker 14H6 had a close linkage with peanut pod rot which considered being the practical application value. 48 pairs of InDel markers were also used to screen American germplasm resources and 15 of 48 InDel markers showed polymorphism among the germplasms with the average polymorphic rate of 90.67%. The results of Pearson correlation analysis and stepwise multiple linear regression analysis showed that only 2 InDel (InDel-004, InDel-020) markers were found to be significantly correlated with peanut pod rot resistance. And the proportions of phenotypic variance explained by InDel-004 and InDel-020 were 5.1% and 5.9%, respectively, for what these two markers were not validated by F2 population due to low practical application value expectation. This study not only confirmed the resistance of peanut germplasm from different sources but also provided excellent materials forgenetic improvement of peanut disease resistance by rational utilization of germplasm. And furthermore, the acquisition of molecular markers related to peanut pod rot resistance could provide an important reference for molecular assisted selection breeding and the excavation of disease-related genes.
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Received: 26 September 2018
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Corresponding Authors:
liulifeng@hebau.edu.cn
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