Abstract：The efe gene encoding ethylene-forming enzyme was PCR amplified from Pseudomonas syringae pv. glycinea ICMP2189. The efe gene was cloned to expression vector pET28a, yielding recombinant plasmid pET28a-efe. Then plasmid pET28a-efe was introduced into Escherichia coli BL21(DE3). SDS-PAGE showed that ethylene-forming enzyme with molecular weight of 45 kDa was effectively expressed in E. coli. Gas chromatography analysis demonstrated that the E.coli strain carrying the efe gene could efficiently produce ethylene.
