Abstract：Abstract: The human erythropoietin gene (hEPO), containing all exons and introns of the hEPO gene except intron 1, was cloned by using PCR technique and synthesized. The hEPO gene was subcloned into the pIRES2-AcGFP1 to construct the eukaryotic expression vector to co-expression hEPO and AcGFP1. The vector was transfected into Chinese hamster ovary cells (CHO) and goat fibroblast by lipidosome transfection. The results showed that CHO and goat fibroblasts appeared bright fluorescence 48h after transfection. Screened by G418, expression cells were obtained. This indicated that the eukaryotic expression vector of hEPO gene with reporter gene has been constructed and expressed in CHO cells and goat fibroblasts. The results paved the way for detecting whether hEPO gene had been transduced into cells, as well as screening positive cells.
陈阿琴 俞颂东 黄光菊 王争光. 人促红细胞生成素基因和AcGFP1共表达真核表达载体的构建及鉴定[J]. , 2007, 15(4): 0-.
. Construction and Identification of Eukaryotic Expression Vector to Co-expression Human Erythropoietin Gene and AcGFP1. , 2007, 15(4): 0-.
