Research Progress and Application of Genetic Transformation Technology by Pollen-tube Pathway
GE Bao-Zhi1,2, SONG Jie1, PENG Lv-Chun1, LI Wei3, GUAN Wen-Ling2,*, ZHANG Lu1,*
1 Flower Research Institute/National Engineering Research Center for Ornamental Horticulture/Key Laboratory of Flower Breeding of Yunnan province, Yunnan Academy of Agricultural Sciences, Kunming 650205, China; 2 School of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China; 3 Yunnan Jicheng Landscape Technology Co., Ltd., Honghe 652339, China
Abstract：The genetic transformation technology of pollen-tube pathway, as a commonly used method for genetic transformation, offers advantages such as simplicity of operation, independence from genotype, and the ability to directly obtain transgenic seeds. Despite being influenced by factors such as external environmental conditions, the natural flowering period of plants, and limited flowering frequency, the pollen tube pathway genetic transformation technique remains highly applicable to a wide range of plants, especially those that are genotype-dependent. This article reviewed the origin, classification, and characteristics of this method, discussed the main factors affecting its genetic transformation efficiency and the progress in understanding its transformation mechanisms, and compareed it with the Agrobacterium-mediated genetic transformation method. Finally, the current applications, existing issues, and future prospects of the pollen tube pathway method were elaborated, providing theoretical references for establishing or optimizing a rapid and highly efficient plant genetic transformation technique via the pollen tube pathway.
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