Research Progress of Rapid Polymerase Chain Reaction Equipment
LI Jia-Le1, LIN Sheng-Hao1, XU Wen-Tao1,2,*
1 College of Food Science and Nutritional Engineering,China Agricultural University, Beijing 100083, China; 2 Department of Nutrition and Health, China Agricultural University, Beijing 100083, China
Abstract：Polymerase chain reaction (PCR) is widely used in many fields because of its unique advantages. Traditional PCR technology takes a long time, which is generally more than 60 min, so a method of fast PCR is proposed. This paper focuses on the fast PCR reaction device. According to the heat transfer mode, the contact (continuous flow, fixed chamber, oscillating swing) and non-contact (infrared, laser, metal nanostructure, microwave, electromagnetic induction) thermal cycling instruments are introduced and analyzed respectively. Finally, the operation integration of rapid PCR technology, the further research and development of metal nanostructures and the commercial production of rapid thermal cycling instrument are discussed and prospected. This review provides a reference for the research and development of low-cost, miniaturized, automatic and high-throughput heat cycle instrument in the future.
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