Effects of Overexpression of St536 Gene on Cellulose Accumulation in Nicotiana tabacum
SA Shi-Juan1, YIN Qian1, WU Han-Yu1, XI Yun-Feng1, ZHENG Rui1,2, YAO Xin-Ling1,2,*
1 Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia, Ningxia University, Yinchuan 750021, China; 2 Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resource in the Western China, Ningxia University, Yinchuan 750021, China
Abstract：Mitochondrial membrane-binding proteins (MMBPs) encoded by nucleus genes involve in cellulose accumulation. However, more MMBPs need to be identified and characterized to disclose its role in cellulose accumulation metabolism. In this study, St536 (ID: PGSC0003DMG400012536), which was screened from the previous transcriptome analysis and encoding membrane-binding protein, was cloned in potato (Solanum tuberosum). Sequence analysis showed that the ORF of St536 gene was 648 bp, encoded 215 amino acid. Subcellular localization showed that the protein encoded by St536 gene was detected in the mitochondrial membrane. St536 overexpression vector was constructed and transformed into tobacco (Nicotiana tabacum) to obtain St536 overexpression lines of tobacco. Mophological assay and physiological analysis on the St536 overexpression lines showed that cellulose accumulation and the ratio of stomatal and guard cell relative vertical length (GL/SL) in the overexpression lines reduced 20% and 17%, respectively, comparing with wild type (WT). Stomatal conductance and photosynthetic rates in the overexpression lines were 2.4 and 2.5 times of the wild type, respectively. The result indicates that St536 overexpression decreased cellulose accumulation, lead to stomatal open and close easier, and enhanced stomatal conductance and photosynthesis rate. The study confirmed that St536 was involved in leaf cellulose accumulation, and provides basic data for further revealing the mechanism of cellulose accumulation.
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