Fitness of the Glyphosate-resistant Second Backcross Generation (BC2F1) Between Glyphosate-resistant Transgenic Oilseed Rape (Brassica napus) and Four Populations of Wild Brassica juncea
LIU Long-Nan, LI He-Wei, ZHANG Hua, QIANG Sheng, SONG Xiao-Ling*
Weed Research Laboratory, College of Life Sciences of Nanjing Agricultural University, Nanjing 210095, China
Abstract：With the rapid development of genetically modified technology, the environmental biosafety of transgene introgression into wild relatives has attracted much attention. To evaluate whether the transgene endowing glyphosate-resistance to Brassica napus could successfully introgress to the different populations of wild Brassica juncea, the fitness of the glyphosate-resistant second backcross generations BC2mF1 and BC2pF1 (m and p represent wild B. juncea as maternal plants and paternal plants, respectively) between 4 populations of wild B. juncea (Jurong, Nantong, Xining, Xi'an) and glyphosate-resistant oilseed rape was studied in the greenhouse and field. Vegetative growth indicators and reproductive growth indicators of these 4 second backcross generations were measured and used to calculate their composite fitness. Furthermore, the differences of the composite fitness between the second backcross generations and wild B. juncea were analyzed. The results showed that the composite fitness of all BC2mF1 and BC2pF1 were similar with that of their respective wild B. juncea under greenhouse conditions. Similarly, in the field, the BC2mF1 of Jurong, Xining, and Xi'an and BC2pF1 of Nantong and Xi'an were equivalent in composite fitness to their respective wild B. juncea, However, composite fitness of BC2pF1 of Jurong, Xining and BC2mF1 of Nantong surpassed that of their respective wild B. juncea progenitor. In conclusion, the glyphosate-resistant BC2mF1 and BC2pF1 of transgenic oilseed rape and the 4 wild B. juncea had a strong survival ability and potential possibility of establishing populations in the field. It was necessary to prevent initial gene flow and introgression to backcrosses between the herbicide-resistant transgenic oilseed rape and wild B. juncea upon commercial release of the transgenic crop. The results will provide data on the possible ecological consequences of the introgress of transgene from glyphosate-resistant transgenic oilseed rape into different populations of wild B. juncea.
刘龙男, 李鹤卫, 张华, 强胜, 宋小玲. 抗草甘膦转基因油菜与4个种群野芥菜抗性回交2代(BC2F1)的适合度[J]. 农业生物技术学报, 2020, 28(8): 1379-1389.
LIU Long-Nan, LI He-Wei, ZHANG Hua, QIANG Sheng, SONG Xiao-Ling. Fitness of the Glyphosate-resistant Second Backcross Generation (BC2F1) Between Glyphosate-resistant Transgenic Oilseed Rape (Brassica napus) and Four Populations of Wild Brassica juncea. 农业生物技术学报, 2020, 28(8): 1379-1389.
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