Abstract：Abstract: Flower development has vital significance for the reproduction of sunflower offspring., and the MADS-box gene plays an important role in this process. In order to explore the function of the MADS-box gene Transcription factor 6 in sunflower (Helianthus annuus L.)，Hatrans 6 gene was cloned from sunflower (XM_022141206). And it is analyzed systematically. The results of gene sequence information analysis showed that Hatrans 6 had typical mads-box and k-box conserved domains, and was a candidate member gene of MADS-box. Phylogenetic tree analysis showed that Hatrans 6 gene had the closest homologous relationship with Agamous-like 13 gene and Agamous-like 6 gene of Arabidopsis thaliana L. The expression pattern analysis of qRT-PCR indicated that Hatrans 6 gene was highly expressed in flower and immature seed. The higher and stable expression level was observed from floral organ primordial stage to 5 d after flowering, and reached its top level at floral organ primordial stage. In addition, the results are shown Hatrans 6 gene was expressed in bract, crown, petal, stamen, pistil and ovary on 5 days before and the very day of flowering, in ovary, followed by bract. The results of this study suggested that Hatrans 6 gene could play an important role in regulating floral development and early fruit development of sunflower, providing basic datee for further investigation of the functional of Hatrans6 gene.
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