Abstract：Awang sheep (Ovis aries), as a special and precious sheep breed resource in the Tibet Plateau, has unique flavors, high-quality meat production and strong plateau adaptability. To prevent the decline of Awang sheep in stock and to protect local germplasm resources, this study analyzed the effects of different follicle-stimulating hormone (FSH) sources and its doses of FSH, superovulation times and the estrus history on the effect of superovulation in Awang sheep by synchronous estrus, superovulation and surgical embryo recovery. The results showed that domestic FSH had the best effect on superovulation in Awang sheep. The average number of corpus luteum and average effective embryos in domestic FSH group were significantly higher than those in the imported FSH group and the domestic + imported FSH group (P<0.05). There was no significant difference in the average number of available embryos and embryo recovery rate (P>0.05). The 270 and 500 IU of domestic FSH had the same effect on superovulation in Awang sheep, and had no significant effect on the superovulation effect of Awang sheep weighing(>50 kg and 40~50 kg), respectively. There was no significant effect of oestrus history on the effect of superovulation. In addition, the total number of corpus luteum and the number of recovered embryos in the first superovulation group were extremely significant higher than those in the multiple superovulation (P<0.01). In conclusion, 270 IU of domestic FSH had the best effect on superovulation in Awang sheep, which can be used as a reference for the conservation and propagation of local Awang sheep.
李涛, 王幸栓, 虞莲, 杨志勇, 林耀民, 高志敏, 李建强, 雷安民. FSH来源及其剂量对西藏阿旺绵羊超数排卵效果的比较[J]. 农业生物技术学报, 2023, 31(9): 1890-1898.
LI Tao, WANG Xing-Shuan, YU Lian, YANG Zhi-Yong, LIN Yao-Min, GAO Zhi-Min, LI Jian-Qiang, LEI An-Min. Comparative on Superovulation Effects with FSH Source and Its Dosage on Tibet Awang Sheep (Ovis aries). 农业生物技术学报, 2023, 31(9): 1890-1898.
[1] 陈天宝, 熊朝瑞, 骆佳锐, 等. 2011. 阿旺绵羊生产性能研究[C]//. 2011. 中国羊业进展论文集, 中国畜牧业协会, pp. 236-237. (Chen T B, Xiong C R, Luo J R, et al.2011. Research on the production performance of Awang sheep[C]//. Proceedings of the progress of China's sheep industry, China Animal Agriculture Association, pp. 236-237.) [2] 德庆卓嘎, 洛桑崔成, 格桑加措, 等. 2019. 西藏阿旺绵羊生产性能研究[J]. 当代畜禽养殖业, (4): 3-6. (Deqing Z G, Luosang C C, Gesang J C, et al. 2019. Research on the production performance of Awang sheep in Tibet[J]. Contemporary Livestock and Poultry Breeding, (4): 3-6.) [3] 德庆卓嘎. 2013. 西藏阿旺绵羊遗传资源调查研究[D]. 硕士学位论文, 中国农业科学院, 导师: 马月辉, pp. 1-2; 21-22. (Deqing Z G.2013. Investigation and re‐search on sheep genetic resources in Awang, Tibet[D]. Thesis for M.S., Chinese Academy of Agricultural Sciences, Supervisor: Ma Y H, pp. 1-2; 21-22.) [4] 丁红, 玛依拉, 杨冬梅等. 1998. 促卵泡素和年龄对绵羊超数排卵效果的影响[J]. 中国畜牧杂志, 34(03): 18-20. (Ding H, Ma Y L, Yang D M, et al.1998. Effects of follicle stimulating hormone and age on superovulation in sheep[J]. Chinese Journal of Animal Science, 34(03): 18-20.) [5] 郭延华, 万鹏程, 刘长彬. 2017. 肉用绵羊超数排卵效果实例分析[J]. 新疆农业科学, 54(8): 9. (Guo Y H, Wan P C and Liu C B.2017. A case study on the effect of superovulation in meat sheep[J]. Xinjiang Agricultural Science, 54(8): 9.) [6] 李俊, 桑滋润, 李铁栓, 等. 2005. 杜泊羊胚胎移植试验[J]. 畜牧与兽医, 37(2): 2. (Li J, Sang Z R, Li T S, et al.2005. Experiment of dorper sheep embryo transfer[J]. Animal Husbandry and Veterinary Medicine, 37(2): 2.) [7] 李雪松, 吕津, 桑润滋, 等. 2005. 重复超排对奶山羊超排效果的影响[J]. 黑龙江动物繁殖, 13(2): 8-9. (Li XS, L J, Sang R Z, et al.2005. The effect of repeated superovulation on the superovulation effect of dairy goats[J]. Heilongjiang Journal of Animal Reproduction, 13(2): 8-9.) [8] 骆佳锐, 陈天宝, 张红平. 2012. 阿旺绵羊羊毛品质和生产性能的测定[J]. 中国草食动物科学, (S1): 413-415. (Luo J R, Chen T B, Zhang H P, et al. 2012. Determination of wool quality and production performance of Ngawang sheep[J]. China Herbivore Science, (S1): 413-415.) [9] 田秀娥, 王永军, 付明哲, 等. 2018. 进口和国产激素处理杜泊羊的发情和超排效果比较[J]. 家畜生态学报, 39(10): 4. (Tian X E, Wang Y J, Fu M Z, et al.2018. Comparison of estrus and superovulation effects in Dorper sheep treated with imported and domestic hormones[J]. Journal of Livestock Ecology, 39(10): 4.) [10] 扎西, 央金, 普布次仁, 等. 2011. 阿旺绵羊种质特性研究报告[J]. 草食家畜, (3): 28-30. (Tashi, Yang J, Pubu T, et al. 2011. Research report on germplasm characteristics of Awang sheep[J]. Herbivorous Livestock, (3): 28-30.) [11] 杨昇, 冯建忠, 张金龙, 等. 2005. 波尔山羊的超数排卵及影响因素的分析[J]. 中国畜牧杂志, 41(01): 39-41. (Yang Y, Feng J Z, Zhang J L., et al.2005. Analysis of superovulation and influencing factors in goats[J].Chinese Journal of Animal Science, 41(01): 39-41.) [12] 张果平, 王金文, 王德芹, 等. 2008. 影响杜泊绵羊超数排卵效果的因素分析[J]. 江苏农业学报, 24(3): 293-297. (Zhang G P, Wang J W, Wang D Q, et al.2008. Analysis of factors affecting the effect of superovulation in Dorper sheep[J]. Journal of Jiangsu Agricultural Science, 24(3): 293-297.) [13] 张锁林, 王述宇. 2001. 波尔山羊超数排卵方案的研究[J]. 中国草食动物科学, 3(04): 10-14. Zhang S L, Wang S Y. 2001. Study on superovulation plan of Boer goat[J]. China Herbivorous science, 3(04): 10-14. [14] Alabart J L, Folch J, Fernández-Arias A, et al.2003. Screening of some variables influencing the results of embryo transfer in the ewe[J]. Theriogenology, 59(5-6):1345-1356. [15] Armstrong D T.1983. Factors influencing success of embryo transfer in sheep and goats[J]. Theriogenology, 19(1): 31-42. [16] Bari F, Khalid M, Haresign W, et al.2000. Effect of mating system, flushing procedure, progesterone dose and donor ewe age on the yield and quality of embryos within a MOET program in sheep[J]. Theriogenology, 53(3): 727-742. [17] Bari F, Khalid M, Haresign W, et al.2003. Factors affecting the survival of sheep embryos after transfer within a MOET program[J]. Theriogenology, 59(5-6): 1265-1275. [18] Bartlewski P M, Baby T E, Giffin J L.2011. Reproductive cycles in sheep[J]. Animal Reproduction Science, 124(3-4): 259-268. [19] Bartlewski P M, Murawski M, Schwarz T, et al.2017. Effect of altering the intervals between consecutive superovulatory doses of porcine follicle-stimulating hormone on ovarian responses and embryo yields in anestrous ewes[J]. Animal Reproduction Science, 180: 44-49. [20] Bartlewski, P M, Seaton P, Oliveira M F, et al.2016. Intrinsic determinants and predictors of superovulatory yields in sheep: Circulating concentrations of reproductive hormones, ovarian status, and antral follicular blood flow[J]. Theriogenology, 86(1): 130-143. [21] Behringer R, Gertsenstein M, Nagy K V, et al.2018. Administration of gonadotropins for superovulation in mice[J]. Cold Spring Harbor Protocols,(1): prot092403. [22] Ben-Menahem and David.2018. Preparation, characterization and application of long-acting FSH analogs for assisted reproduction[J]. Theriogenology, 112: 11-17. [23] Bondurant R H, Skirrow S, Anderson G B, et al.1984. Nonsurgical collection of blastocysts from dairy goats[J]. Theriogenology, 22(4): 423-431. [24] Campbell B K, Dobson H, Baird D T, et al.1999. Examination of the relative role of FSH and LH in the mechanism of ovulatory follicle selection in sheep[J]. Journal of Reproduction and Fertility, 117(2): 355-367. [25] Floresfoxworth G, Mcbride B M, Kraemer D C, et al.1992. A comparison between laparoscopic and transcervical embryo collection and transfer in goats[J]. Theriogenology, 37(1): 213-213. [26] Forcada F, Amer-Meziane M A, Abecia J A, et al.2011. Repeated superovulation using a simplified FSH/eCG treatment for in vivo embryo production in sheep[J]. Theriogenology, 75(4): 769-776. [27] Holtz W.1996. Embryo transfer in the goat-a review[J]. Dtsch Tierarztl Wochenschr, 103(8-9): 293-297. [28] Kenichiro S, Atsushi I, Yojiro Y, et al.2018. Effect of a single epidural administration of follicle-stimulating hormone via caudal vertebrae on superstimulation for in vivo and in vitro embryo production in Japanese black cows[J]. Journal of Reproduction & Development, 64(4): 451-455. [29] Mckelvey W, Robinson J J, Aitken R P, et al.1986. Repeated recoveries of embryos from ewes by laparoscopy[J]. Theriogenology, 25(6): 855-865. [30] Nowshari M A, Holtz W.1995. In vitro culture of goat morulae to blastocysts before freezing[J]. Theriogenology, 44(7): 983. [31] Seekallu S V, Toosi B M, Duggavathi R, et al.D2010. Ovarian antral follicular dynamics in sheep revisited: Comparison among estrous cycles with three or four follicular waves[J]. Theriogenology, 73(5): 670-680. [32] Souza-Fabjan J M G, Da Rosa R M, Balaro M F A, et al.2017. Effect of different hormonal combinations on follicular wave emergence and superovulatory response in sheep[J]. Theriogenology, 103: 24-29. [33] Zhao E, Qian Y, Zhang N, et al.2013. Mitochondrial DNA diversity and the origin of Chinese indigenous sheep[J]. Tropical Animal Health and Production, 45(8): 1715-1722. [34] Zhao Y, Zhao E, Zhang N, et al.2011. Mitochondrial DNA diversity, origin, and phylogenic relationships of three Chinese large-fat-tailed sheep breeds[J]. Tropical Animal Health & Production, 43(7): 1405-1410. [35] Zuchthygiene B G, Baril G, Casamitjana P, et al.1989. Embryo production, freezing and transfer in Angora, Alpine and Saanen goats[J]. Zuchthygiene, 24(3): 101-115.
