Single-cell Transcriptome Sequencing Technology and Its Application in the Field of Animal Science
LOU Ji-Ying1, GUO Qi-Xin1, BI Yu-Lin1, JIANG Yong1, WANG Zhi-Xiu1, CHEN Guo-Hong1,2, BAI Hao2*, CHANG Guo-Bin1,2*
1 College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 2 Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China
Abstract：Single-cell sequencing is a new research area, which mainly includes transcriptome sequencing, whole genome sequencing, and epigenomic sequencing. Single-cell RNA-sequencing (scRNA-seq) refers to a new technique for high-throughput sequencing and analysis of RNA at the single cell level. Recently, single- cell related experimental technology has developed rapidly and has been widely used in the field of biology and medicine, mainly for the study of biological evolution process, tissue and organ development, tumor disease occurrence and precision medical diagnosis. However, few studies were reported in the field of animal science research and its application, currently, most of them focus on the field of animal physiology and evolutionary development. This article summarized the technical methods of single-cell transcriptome sequencing and its application in the field of animal science. This review provides a theoretical basis for the development of single-cell transcriptome sequencing technology and its application in the field of animal science.
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