Abstract：Infectious hematopoietic necrosis virus (IHNV) infection causes much higher morbidity and mortality in small-sized rainbow trout (Oncorhynchus mykiss) than in large-sized fish. In order to explore the possible mechanism of this difference from the perspective of intestinal and hepatic immune response and microecological health of fish, 2 sizes of rainbow trout fingerling were selected from the same cage culture area where IHNV infection was detected. Using qPCR and 16S ribosomal DNA identification (16S rDNA) comparative analysis the Toll-like receptor 2/7 (TLR2/7), interleukin-8/18 (IL-8/18), interferon (IFN), interferon regulatory factor 9 (IRF9), Myxovirus resistant 1 (MX1) and vipern (VIG-1) genes were involved in the immune response mechanism and intestinal microflora differences. The results showed that among the 8 genes examined, TLR7 and MX1 were significantly higher expressed in the intestine of small-sized rainbow trout than large-sized fish (P<0.05), IL-8 was significantly higher expressed in the liver of small-sized rainbow trout than the large-sized (P<0.05), and the intestinal microbial diversity of large-sized rainbow trout was higher than that of small-sized fish. The abundance of Actinobacteria and Bacillus in the intestine of large- size rainbow trout (Oncorhynchus mykiss) fingerling was significantly higher than that of small-size fish (P<0.05), and the abundance of Serratia in the intestine of small-size rainbow trout was significantly higher than that of large-size fish (P<0.05). In conclusion, small-sized fish may have higher susceptibility to pathogenic stressors such as viruses that may be present in the environment, the intestinal micro-ecosystem was less stable compared to large-sized, and the abundance of beneficial bacteria present in the intestine of large-sized rainbow trout that may be related to a higher disease resistance. The results of this study elucidate the differences in immune genes and intestinal microorganisms of different sizes of rainbow trout, and provide theoretical support for the prevention and treatment of IHNV from immune genes and intestinal microbial regulation.
海强, 王建福, 康维国, 吕娜娜, 刘钰, 刘哲, 成述儒. 2 种规格虹鳟免疫相关基因表达及肠道微生物菌群差异分析[J]. 农业生物技术学报, 2023, 31(5): 1043-1052.
HAI Qiang, WANG Jian-Fu, KANG Wei-Guo, LYU Na-Na, LIU Yu, LIU Zhe, CHENG Shu-Ru. Analysis of Immune-related Gene Expression and Differences in Gut Microflora Between Two Sizes of Rainbow Trout (Oncorhynchus mykiss). 农业生物技术学报, 2023, 31(5): 1043-1052.
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