Abstract：Long-chain non-coding RNA (lncRNA) has become an important participant in almost all gene functions and regulatory levels. In order to reveal the potential effect of lncRNA on the milk yield of horses (Equus caballus) in early lactation, 8 healthy Kazakh horses of 3~4 years old with similar body shape and early lactation were selected in this study, and they were divided into high milk yield group (H) and low milk yield group (L) according to their milk yield. The non-coding RNA in the milk of 2 groups of horses was sequenced by transcriptome sequencing technology. Through the significant enrichment analysis of GO and Pathway functions, the main biological functions of differentially expressed genes, biochemical metabolic pathways and signal pathways involved were determined. According to P<0.05 and FC≥1, the differentially expressed lncRNA was screened out, and the function of the differentially expressed lncRNA was further studied. The results showed that a total of 30 differentially expressed lncRNA (up-regulated 16, down-regulated 14) were found in Kazakh horse milk of high-yield group and low-yield group in early lactation. Genes related to breast development and lactation, such as vascular endothlial growth factor, (VEGFD), phosphatidylinositol glycan, class A (PIGA), and aconitase closing odor (ACO1) were screened out. The results showed that lncRNA may regulate the process of lactation or mammary gland development by targeting genes related to mammary gland development and lactation. This study provides reference for the application of lncRNA in horse breeding.
马应国, 梁金超, 马海玉, 刘玲玲, 刘武军. 基于转录组测序技术对哈萨克马产奶量相关差异表达 lncRNA 的研究[J]. 农业生物技术学报, 2023, 31(5): 1001-1012.
MA Ying-Guo, LIANG Jin-Chao, MA Hai-Yu, LIU Ling-Ling, LIU Wu-Jun. Study of Differentially Expressed LncRNA Related to Milk Production in Kazakh Horses (Equus caballus) Based on Transcriptome Sequencing Technology. 农业生物技术学报, 2023, 31(5): 1001-1012.
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