Effects of Five Immunopotentiators on the Expression of Some Inflammatory and Apoptosis-related Factors in Thymus Tissues of Shaoxing Ducklings (Anas platyrhynchos)
1 State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China;
2 Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3 Zhejiang Guowei Technology Co., Ltd., Zhuji 311800, China
Abstract：The misuse of antibiotics seriously imperils the development of duck husbandry and even endangers human health. Therefore, it is urgent to find reliable alternatives to antibiotics. Immunopotentiator can effectively increase the ability of specific immune responses to enhance the defense against microorganisms or pathogens. The aim of this study was to investigate the effects of 5 different immunopotentiators on the thymus apoptosis and immune-related gene expression in Shaoxing ducklings (Anas platyrhynchos). The 150 1-day-old Shaoxing ducklings were randomly divided into 6 groups, and saline, chlorogenic acid, β -D-glucan, astragalus flavone, nonmethylated CpG DNA and chicken immunoglobulin G (IgG), were injected subcutaneously once daily for 3 d, respectively. At the age of 18 d, the mRNA and protein expression of apoptosis and immune-related genes were detected by qPCR and Western blot. The results showed that compared with control group, the mRNA and protein levels of thymus B-cell lymphoma 2 (Bcl2) were significantly decreased during injecting chlorogenic acid, β-D-glucan, astragalus flavone, nonmethylated CpG DNA and chicken IgG, respectively (P＜0.05). And the expression levels of thymus Caspase 3 showed significantly up-regulated in some extent (P＜0.05). In addition, the expression level of thymus inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX2) were increased in some extent during injecting 5 different immunopotentiators, respectively. Meanwhile, compared with control group, the levels of interferon-α (IFN- α), interferon-β (IFN-β), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), retinoic acid-inducible gene-I (RIG-I), toll-like receptor 3 (TLR3) and toll-like receptor 7 (TLR7) mRNA expression were up-regulated in some extent during injecting 5 different immunopotentiators, respectively. Taken together, chlorogenic acid, β -D-Glucan, astragalus flavone, nonmethylated CpG DNA and chicken IgG could be used as immunopotentiators to regulate duck innate immune. This study provides new insight to prevent the duck disease from pathogen infections, and provides reference for reliable alternatives to antibiotics in animal production.
顾天天, 李国勤,许文武,田勇,李柳萌,陈斌丹,曾涛,陈黎,陶争荣,沈军达,卢立志. 五种免疫增强剂对绍兴雏鸭胸腺组织部分炎症和凋亡相关因子表达的影响[J]. 农业生物技术学报, 2022, 30(7): 1339-1347.
GU Tian-Tian,LI Guo-Qin,XU Wen-Wu,TIAN Yong,LI Liu-Meng,CHEN Bin-Dan,ZENG Tao, CHEN Li,TAO Zheng-Rong,SHEN Jun-Da,LU Li-Zhi. Effects of Five Immunopotentiators on the Expression of Some Inflammatory and Apoptosis-related Factors in Thymus Tissues of Shaoxing Ducklings (Anas platyrhynchos). 农业生物技术学报, 2022, 30(7): 1339-1347.
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