Analysis of the Fertility Sensitivity to Photoperiods and Temperatures of Duration of Cultivation of CMS-WA in Rice (Oryza sativa)
LIN Qiang1,2, LUO Xi1,2, XIE Hong-Guang1,2, HE Wei1,2, WANG Ying-Heng1,2, CAI Qiu-Hua1,2, ZHANG Jian-Fu1,2,*
1 Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350018, China; 2 State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Academy of Agricultural Sciences /Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs /Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology, China/South China Bases of National Key Laboratory of Hybrid Rice for China/National Rice Engineering Laboratory of China, Fuzhou 350003, China;
摘要水稻(Oryza sativa)细胞质雄性不育(cytoplamic male sterility, CMS)系花粉育性不同程度的波动,直接影响杂交稻种子纯度。为了研究水稻野败型细胞质雄性不育(cytoplamic male sterility of wild abortion, CMS-WA)系育性的光温敏感性,本研究设置4个播栽期,4个不同光照长度处理,选择3个微效恢复基因数量不同的野败型(wild abortion type, WA)不育系'珍汕97A'、'龙特浦A'和'京福1A',以嵌合颖花率(rate of florets with fertile anthers)和黑染花粉率(dark pollen rate by I2-IK dyed)为育性指标,应用回归模型分析播栽期温度及光照长度对不育系花粉育性的影响。结果表明,光温互作对不育系中微效恢复基因的表达有重要的影响,随着播栽温度的上升,3个不育系的黑染花粉率和嵌合颖花率总体上均表现上升趋势,'珍汕97A'、'京福1A'的花粉育性对光照长度没有明显的反应,而光照长度对'龙特浦A'的效应达显著水平(P<0.05)。'龙特浦A'可归类为温光并进型,光照长度作用随播栽期温度同时发生互促效应,温光并进型不育系采取早繁早制的措施,种子纯度高;而在选育和提纯上,应在高播栽期温度短日照的条件下进行,及早剔除不合格单株或株系。'珍汕97A'和'京福1A'可归类为温度敏感型,光照长度效应不明显,因而低播栽期温度下繁殖和制种,高播栽期温度下选育和提纯,效果较好。本实验结果对不育系原种提纯、选育改良及繁殖制种具有指导和参考作用。
Abstract：The various degrees sterility fluctuation of CMS (cytoplasmic male sterility) lines directly affects the seeds purity of hybrid rice (Oryza sativa). For analyzing the fertility sensitivity by photoperiods and temperatures of duration of cultivation to CMS-WA (cytoplamic male sterility of wild abortion), 4 temperatures of duration of cultivation and 4 photoperiods were selected to study male fertility changes of 3 CMS-WA lines ('Zhenshan 97A', 'Longtepu A' and 'Jinfu 1A') which had different numbers of minor effect restoring gene. The dark pollen rate by I2-IK (mixed solution of I2 and IK) dyed and rate of florets with fertile anthers were analyzed by linear regression model. For constructing predictive analysis models, and exploring the genetic basis of dark pollen rate by I2-IK dyed and rate of florets with fertile anthers caused by the interaction between temperatures of duration of cultivation and photoperiods and restoring gene with minor effect. The results showed that dark pollen rate by I2-IK dyed and rate of florets with fertile anthers of male sterile lines were importantly affected by the interaction between photoperiods and temperatures of duration of cultivation. Generally, with temperature rising, dark pollen rate by I2-IK dyed and rate of florets with fertile anthers of CMS lines were increasing. With photoperiod increasing, significant impact was observed in 'Longtepu A', but not in 'Jingfu 1A' and 'Zhenshan 97A'. So, 'Longtepu A' should be classified as photo-thermo-sensitive type, it meant that 'Longtepu A' had mutual promotion effect by photoperiods and temperatures of duration of cultivation, and early season propagation and early season production were priority adopted to ensure the purity of seeds. Breeding and purification of photo-thermo-sensitive type CMS lines, should be conducted at short photoperiods and high temperature of duration of cultivation. Thereby the unqualified individual plant or lines could be eliminated as soon as possible. 'Zhenshan 97A' and 'Jingfu 1A' belonged to thermo-sensitive type, and had no obvious photoperiod effect. Low temperature of duration of cultivation was large contribution to propagation and production seed, and high temperature was beneficial to breeding and purification of thermo-sensitive type CMS lines. The result could provide reference basis for breeding, purification, propagation and reproduction of male sterile lines.
林强, 罗曦, 谢鸿光, 何炜, 王颖姮, 蔡秋华, 张建福. 水稻CMS-WA育性的光温敏感性分析[J]. 农业生物技术学报, 2020, 28(1): 22-31.
LIN Qiang, LUO Xi, XIE Hong-Guang, HE Wei, WANG Ying-Heng, CAI Qiu-Hua, ZHANG Jian-Fu. Analysis of the Fertility Sensitivity to Photoperiods and Temperatures of Duration of Cultivation of CMS-WA in Rice (Oryza sativa). 农业生物技术学报, 2020, 28(1): 22-31.
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