Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)
ZHAO Zhi-Dong1, TIAN Hong-Shan1, JIANG Yan-Yan1, SHI Bin-Gang1, LIU Xiu1, LI Xu-Peng1, WANG Deng-Zhe1, CHEN Jin-Lin2, HU Jiang1, *
1 College of Animal Science and Technology, Gansu Agricultural University/Gansu Key Laboratory of Herbivorous Animal Biotechnology, Lanzhou 730070 ,China; 2 Nagqu Prefecture Grassland Station, Tibet Autonomous Region, Nagqu 852100, China
Abstract：Long chain acyl-CoA synthetase 1 (ACSL1) plays a key role in the synthesis of triglycerides, phospholipids and cholesterol esters and the oxidation of fatty acids. The Bos grunniens milk has rich nutrition and higher milk fat. However, there are few studies on the genetic mechanism of milk quality traits. In this study, Gannan yak was used as the research object, and the polymorphic loci of the promoter region of yak ACSL1 gene were detected by DNA sequencing, and the association analysis were performed to evaluate the effects of different haplotypes combination and genotypes on milk quality traits. This study found that 3 SNPs in the promoter region of the ACSL1 gene in the Gannan yak population, which were g.2079A>T, g.2409G>A and g.1235_g1236delTG deletion. In the F1 region of the yak ACSL1 promoter, the animals with MN genotypes had significantly higher the milk protein rate than those with genotypes MN (P<0.05). The animals with BB genotypes had significantly higher total solid matter content and milk fat percentage than those with genotypes AA and AB (P<0.05). In the promoter F2 region, the animals with DD genotypes had significantly higher milk fat percentage than those with genotypes CC. 8 haplotypes and 5 diplotypes were constructed, in which the diplotype H4H4 had significantly higher (P<0.05) or extremely significantly higher milk fat content and total solid content than those with other doplotype (P<0.01). Therefore, the mutation in the promoter region of yak ACSL1 gene can be used as a potential genetic marker for the quality traits of Gannan yak milk which also provide theoretical basis for the molecular genetic research of yak milk quality traits.
赵志东, 田宏山, 蒋艳艳, 石斌刚, 刘秀, 李旭鹏, 王登哲, 陈金林, 胡江. 牦牛ACSL1基因启动子多态性及其与乳品质性状的关联分析[J]. 农业生物技术学报, 2019, 27(9): 1596-1603.
ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens). 农业生物技术学报, 2019, 27(9): 1596-1603.
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