Study on the Inhibition Effect of Nano-Mg(OH)2 to Tea Blackspot Disease Pathogenic Fungi Activity
CHEN Rong1, QIU Sai-Fei1, YOU Yu-Xin1, XIE Yong-Xiao1, NIE Dan-Yue1, LI Jia-Xin2, WANG Cai-Cheng1, GUAN Xiong1, PAN Xiao-Hong1,*
1 State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops / Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education / College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract：Fungal diseases of tea are one of the important causes of tea yield reduction. As environmentally friendly antimicrobial materials, nano-materials can be used to inhibit tea fungal diseases. In this study, nano-Mg(OH)2 was synthesized by coprecipitation method, and the inhibitory effect on tea blackspot pathogenic fungi of tea by nano-Mg(OH)2 was studied. The synthesized nano-Mg(OH)2 was characterized by X-ray powder diffraction (XRD), and the size of nano-Mg(OH)2 at (101) direction was 14.5 nm according to the Scherrer equation. Scanning electron microscopy (SEM) was applied to observe the morphology of nano-Mg(OH)2, and it was found that the nanoparticles were regular sheets of structure. Based on the morphological characteristics, internal transcribed spacer (ITS) sequence analysis of ribosomal DNA, ITS-specific primers PCR detection and phylogenetic comparison, it was confirmed that the isolated pathogen was Guignardia mangiferae, which was rarely reported in tea. The water and alkaline condition were set as blank and positive control groups, nano-Mg(OH)2 with different concentrations were used as experimental groups. After plate coating, the growth diameter of fungi was measured by cross-over method and the inhibition rate was calculated after a period of culture. The results showed that nano-Mg(OH)2 could effectively inhibit the growth of tea fungi, the inhibition rate of 5 mg/mL nano-Mg(OH)2 on mycelial growth was 48.78% in 3 days, and the inhibition rate of 50 mg/mL nano-Mg(OH)2 was 100%, which implying that the inhibition rate was increased with the concentration, and the half maximal effective concentration (EC50) of nano-Mg(OH)2 was 7.63 mg/mL. This study provides scientific basis and technical support for the subsequent research and development of safe and effective nano-preparations.
陈蓉, 邱赛飞, 游雨欣, 谢勇啸, 聂丹玥, 李嘉欣, 王财成, 关雄, 潘晓鸿. 纳米氢氧化镁对茶叶黑斑病原真菌活性的抑制效应研究[J]. 农业生物技术学报, 2019, 27(8): 1460-1466.
CHEN Rong, QIU Sai-Fei, YOU Yu-Xin, XIE Yong-Xiao, NIE Dan-Yue, LI Jia-Xin, WANG Cai-Cheng, GUAN Xiong, PAN Xiao-Hong. Study on the Inhibition Effect of Nano-Mg(OH)2 to Tea Blackspot Disease Pathogenic Fungi Activity. 农业生物技术学报, 2019, 27(8): 1460-1466.
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