Abstract：In the previous study, the laboratory isolated and screened a cold-tolerant aerobic denitrification strain Y-12 from long-term flooded winter paddy field. In this study, the characteristics of nitrification, denitrification characteristics and simultaneous nitrification and denitrification characteristics of strain Y-12 at 15 ℃ were studied using single nitrogen source (ammonium (NH4+-N), nitrate (NO3--N) or nitrite (NO2--N)) and different concentrations of mixed nitrogen sources (ammonium and nitrate, or ammonium and nitrite). The results showed that at 15 ℃, when the nitrogen source was ammonium nitrogen, the removal rates of NH4+-N and total nitrogen (TN) were 100% and 46.2%, respectively. When the nitrogen source was nitrate nitrogen, the removal rates of NO3--N and TN were 99.7% and 53.6%, respectively. When the nitrogen source was nitrite nitrogen, the removal rates of NO2--N and TN were 99.9% and 57.7%, respectively. The total nitrogen removal rates of the low concentration mixed nitrogen source (5 mg/L NH4+-N+5 mg/L NO3--N, 5 mg/L NH4+-N+5 mg/L NO2--N) was 50.0% and 52.4%; the total nitrogen removal rates of high-concentration mixed nitrogen (100 mg/L NH4+-N+100 mg/L NO3--N, 100 mg/L NH4+-N+100 mg/L NO2--N) were 31.8% and 24.7%, and both of them could effectively remove ammonium nitrogen, nitrate nitrogen and nitrite nitrogen. The strain could still grow when the pH raised to about 9.0 under mixed nitrogen source conditions. The research proves that strain Y-12 is a cold-tolerant aerobic denitrifying bacteria with certain alkali resistance, which has good advantages and application potential for the treatment of low temperature and alkaline wastewater (especially ammonium-containing nitrogen), provides theoretical basis and alternative strains for the simultaneous treatment of wastewater containing various nitrogen source in the late autumn to early spring.
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