联系我们 加入收藏
 
年期检索 高级检索
33
2025年4月5日 星期六
农业生物技术学报  2019, Vol. 27 Issue (1): 23-33    DOI: 10.3969/j.issn.1674-7968.2019.01.003
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
结球甘蓝BoTCTP基因表达载体的构建及转化芥蓝的研究
王静静1,2, 曹必好1, 陈国菊1 , 陈长明1, 陈清华1, 雷建军1*
1 华南农业大学 园艺学院,广州 510642;
2 德州市农业科学研究院,德州 253015
Study on Construction of Expression Vector of BoTCTP in Cabbage (Brassica oleracea) and Transferring to Chinese Kale (Brassica alboglabra)
WANG Jing-Jing1,2,CAO Bi-Hao1,CHEN Guo-Ju1,CHEN Chang-Ming1,CHEN Qing-Hua1,LEI Jian-Jun1*
1 College of Horticulture, South China Agriculture University, Guangzhou 510642, China;
2 Dezhou Academy of Agricultural Sciences, Dezhou 253015, China
全文: PDF (6344 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 芥蓝(Brassica alboglabra)是十字花科重要蔬菜之一,在栽培过程中常常会遭受各种逆境的严重影响,给芥蓝的生产造成很大的损失。为提高芥蓝对逆境胁迫的抵抗能力,本研究根据结球甘蓝翻译控制肿瘤蛋白基因(translationally controlled tumor protein cloned from Brassica oleracea, BoTCTP)序列设计特异引物,经PCR扩增得到目的片段,然后构建过表达载体,利用农杆菌(Agrobacterium tumefaciens)介导法导入芥蓝中,并对其转基因植株进行了分子生物学鉴定。研究结果显示,4株外源目的基因已经整合到受体基因组中,1株以双拷贝形式存在,另外3株以单拷贝形式存在。转基因植株的长势旺盛,生长速率加快,叶面积较大,在逆境胁迫(高低温, 盐碱, 干旱, 重金属等)下转基因植株对胁迫的耐受性明显优于对照植株,从而表明BoTCTP基因与芥蓝的生长密切相关,并对芥蓝的抗逆性起调控作用。本研究结果为芥蓝抗逆性的进一步研究提供了科学依据。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
王静静
曹必好
陈国菊
陈长明
陈清华
雷建军
关键词 翻译控制肿瘤蛋白基因(TCTP)表达载体芥蓝遗传转化生长速度抗逆性    
Abstract:Chinese kale (Brassica alboglabra) is one of the important vegetables in the Cruciferous family. It is often subjected to various adverse effects during the cultivation process, causing great losses to the production. In order to improve the resistance of Chinese kale to stress, the specific primers were designed according to BoTCTP (translationally controlled tumor protein cloned from Brassica oleracea) in this research. The interest fragment was amplified by PCR, and then the overexpression vector was constructed and transferred into Chinese kale by Agrobacterium tumefaciens mediated. The transformants were confirmed by molecular methods and biological assay. Four transgenic plants were obtained, and then taken to design the experiments associated with growth and different stress, such as high temperature, low temperature, salt, heavy metal and so on. The results showed that the transgenic plants grew faster, stronger and had bigger leaves than non-transgenic plants. In the different stress condition, the transgenic plants had obviously stronger resistance than the control, which showed that the BoTCTP was closely related to the growth and had a regulatory effect on the stress resistance of Chinese kale. These results provide some scientific basis for the future research on the stress resistance of Chinese kale.
    
ZTFLH:  S635.9  
引用本文:   
王静静 , 曹必好, 陈国菊, 陈长明 , 陈清华 , 雷建军. 结球甘蓝BoTCTP基因表达载体的构建及转化芥蓝的研究[J]. 农业生物技术学报, 2019, 27(1): 23-33.
WANG Jing-Jing ,CAO Bi-Hao,CHEN Guo-Ju,CHEN Chang-Ming,CHEN Qing-Hua,LEI Jian-Jun. Study on Construction of Expression Vector of BoTCTP in Cabbage (Brassica oleracea) and Transferring to Chinese Kale (Brassica alboglabra). 农业生物技术学报, 2019, 27(1): 23-33.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.01.003     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I1/23
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn