Abstract：Velvet antler 32 polypeptide of sika deer (Cervus nippon)， which is a kind of peptide extracted from fresh antler, has obvious activity to promote proliferation and induce differentiation for mesenchymal stem cells (MSCs). Both of adipose tissue-derived stem cells (ADSCs) and MSCs come from mesoderm, and they are high-profile in the research of bone injury repair and anti-tumor. In this research, the effect of velvet antler polypeptide on ADSCs proliferation, migration and adhesiveness have been observed. Methyl thiazolyl tetrazolium assay (MTT) showed that OD had no obvious difference between experiment group and control group after 24 h. But experiment group was obviously higher than control group after 48 and 72 h. Wound healing assay showed that the concentration of velvet antler polypeptide was 1 μg/mL, the scratch width was narrower than control group, and when the concentration was 50 or 5 μg/mL, the scratch width was bigger than control group. Trypsinization assay found that adding isotonic trypsin to digest, with the increase in peptide concentration, cell digestion time shortened significantly and cell adhesion decreased gradually. This research showed that antler polypeptide promoted proliferation of ADSCs, a certain dose and time-dependent could promote the proliferation of ADSCs in mouse (Mus musculus), reducing cell adhesion and low concentrations of peptides could promote cell migration and high concentrations were inhibited. The results suggested that velvet peptides could be used to increase the number of seed cells and reduce its adhesion in specific areas. While providing the possibility of facilitating its transfer to the damaged site at the same time, which provides a new idea for bone tumor and injury treatment.
