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2025年8月5日 星期二
  2016, Vol. 24 Issue (4): 530-537    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
STMP在拟南芥分枝发育过程中的功能鉴定
高梦烛1,张昊2,高静2,王凤茹2,董金皋3
1. 河北农业大学 生命科学学院/河北省植物生理和分子病理学重点实验室
2. 河北农业大学
3. 河北农业大学植物分子病理学实验室
Functional Analysis of STMP in the Process of the Branch Development in Arabidopsis thaliana
1, 1, 1,wang fengru1,
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摘要 拟南芥(Arabidopsis thaliana)中含有脂/固醇结合的相关脂转移蛋白结构域(steroidogenic acute regulatory related lipid transfer, START)的膜相关蛋白(START domain containing-membrane related protein, STMP),STMP是START保守域(第84~295位)功能未知的蛋白质,由440个氨基酸组成、具有跨膜片段、属于磷脂酰胆碱转运蛋白。为明确STMP在拟南芥发育调控过程中的作用,深入了解拟南芥发育调控的分子机理,本研究用35S强启动子启动START结构域,构建START结构域的过表达载体,利用农杆菌(Agrobacterium tumefaciens)侵染花序法把STMP的过表达载体转入野生型拟南芥,获得过表达STMP的转基因拟南芥,并用qRT-PCR技术进行验证;分析转基因拟南芥分枝发育状况,明确STMP在拟南芥分枝调控过程中的作用;用qRT-PCR技术分析STMP的时空表达特性;构建STMP和绿色荧光蛋白(green fluorescent protein, GFP)的融合蛋白载体,把此载体转入野生型拟南芥中,对转基因拟南芥中GFP进行荧光观察,从而对STMP进行亚细胞定位。本研究获得了过表达STMP的转基因株系;过表达STMP的转基因拟南芥分枝比野生型明显增多,突变体stmp的分枝减少;STMP的时空表达特性分析表明,茎中STMP的表达量最高,STMP定位在细胞质膜和细胞质中。结果表明STMP可以促进拟南芥的分枝发育,本研究对完善拟南芥分枝调控机制具有重要的意义,可以为植物株型的定向设计提供理论依据。
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高梦烛
张昊
高静
王凤茹
董金皋
关键词 含START结构域的膜相关蛋白(STMP)分枝拟南芥    
Abstract:Arabidopsis thaliana steroidogenic acute regulatory related lipid transfer (START) domain containing membrane related protein (STMP) is a function unknown protein containing transmembrane segments, it has 440 amino acid. From 84nd to 295th amino acids is START conserved domain. There is a phosphatidycholine (PtdCho) binding site in START domain. In order to clarify the role of STMP in the development and regulation of Arabidopsis thaliana, this study used the 35S promoter to construct the START domain, Agrobacterium tumefaciens was used to infect the expression vector of STMP in wild type Arabidopsis thaliana, obtained the transgenic Arabidopsis thaliana and used qRT-PCR technology to verify it; Clear the role of STMP in the process of the regulation of the branch of Arabidopsis thaliana through analysis of the developmental status of the transgenic Arabidopsis thaliana; Analysis of temporal and spatial expression of STMP by qRT-PCR technique; Preliminary analysis of the subcellular localization of STMP was performed through the establishment of STMP-GFP (green fluorescent protein) fusion expression vector and protoplast transformation. In this study, transgenic lines over-expressing STMP were obtained. The expression of STMP in transgenic plants was significantly higher than that in wild type, and the temporal and spatial expression of STMP showed that the expression of STMP in stem was the highest, Green GFP localized on cell membrane and cell cytoplasm. These results showed that STMP promotes the development of the branches in Arabidopsis thaliana, This study has important significance to improve the regulatory mechanism of Arabidopsis thaliana branching, and provide a theoretical basis for the design of directional plant type.
Key wordsSTART domain containing membrane related protein (STMP)    Branching    Arabidopsis thaliana
收稿日期: 2015-09-16      出版日期: 2016-02-24
基金资助:BR调控植物近/远轴极性分化的分子机制
通讯作者: 王凤茹     E-mail: fengruwang@yahoo.com.cn;wfr15931945160@126.com
引用本文:   
高梦烛 张昊 高静 王凤茹 董金皋. STMP在拟南芥分枝发育过程中的功能鉴定[J]. , 2016, 24(4): 530-537.
wang fengru. Functional Analysis of STMP in the Process of the Branch Development in Arabidopsis thaliana . , 2016, 24(4): 530-537.
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http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2016/V24/I4/530
 
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