Abstract：In order to study the quality condition of the spring wheat (Triticum aestivum) in Gansu Province and identify the corresponding quality-affecting factors, the present research, based on the determination of their high molecular weight glutenin subunits (HMW-GS), identified the 1BL/1RS translocation lines using molecular and biochemical detection technologies, as well as the genomic in situ hybridization (GISH) and analyzed gluten cooking features. The result showed that Ganchun 24, Ganchun 22, Wuchun 4 and Wuchun 121 were truly the translocated line and Ganchun 23, Ganchun 20, Yongliang 4 and Heshangtou were not. The result explained why the 4 varieties of translocated lines had high quality HMW-GS without high quality. Comparing the 3 detection technologies, it was found that acid poly acrylamide gel electrophoresis (A-PAGE) and GISH could produce more accurate results in determining 1BL/1RS than molecular detection technology. The gluten cooking features analysis showed that the varieties of 1BL/1RS translocated lines were inclined to swollen and collapse. Ganchun 24, Ganchun 22, Wuchun 4 of the 4 of 1BL/1RS translocated line varieties were middle gluten and had the fine subunit of (5+10), while Wuchun 121 was weak gluten variety and did not have the subunit (5+10). This indicated that the high-quality subunit (5+10) could compensate the negative effect on variety quality caused by 1BL/1RS translocation lines. The result could provide theoretical basis for accurate and effective determination of 1BL/1RS translocation lines, as well as for wheat quality breeding and food processing.
Bao Y, Li X, Liu S, et al. 2009. Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium partial amphiploid resistant to powdery mildew and stripe rust[J]. Cytogenet Genome Res, 126: 390-395.
Dhaliwal A S, Mares D J, Marshall D R. 1987. Effect of the 1B/1R chromosome translocation on milling and quality characteristics of bread wheat. Cereal chemistry[J]. 64(2): 72-76.
Graybosch R A. 2001. Uneasy Unions: Quality effects of rye chromatin transfers to wheat[J]. Journal of Cereal Science, 33: 3-16.
Graybosh R A, Peterson C J, Hansen L E, et al. 1990. Relationship between protein solubility characteristics, 1BL/1RS, high-molacular weight glutenin composition and enduse quality in winter wheat germplasm[J]. Cereal Chemistry, 67: 342-349.
Huang D Y, Khan K. 1997. Quantitative determination of high molecular weight glutenin subunit of hard red spring wheat by SDS-PAGE. I. Quantitive effects of total amounts on breadmaking quality characteristics [J]. Cereal Chemistry, 76: 781-785.
J F Chai, Zhou R H, Jia J Z, et al. 2006. Development and application of a new codominant PCR marker for detecting 1BL/1RS wheat rye chromosome translocations[J]. Plant Breed, 125: 302-304.
Rogers S O BAJ. 1985. Extraction of DNA from milligram amounts of fresh herbarium and mummified plant tissues [J]. Plant Mol Biol., 5: 69-76.
Wieser H, Seilmeir W, Belitz, H D. 1994. Quantitave determination of gliadin subgroups from different wheat cultivars [J]. Cereal Sci, 14: 149-155.
Yu Sh-y, Long H, Yang H, et al. 2012. Molecular detection of rye (Secale cereale L.) chromatin in wheat line 07jian126 (Triticum aestivum L.) and its association to wheat powdery mildew resistance[J]. Euphytica, 186(1): 247-255.
陈加江. 2008. 1BL/1RS易位系小麦谷蛋白和醇溶蛋白亚基组成、含量及其对加工品质性状的影响[D].山东农业大学. (Chen J-J. 2008. Comparative investigations of gliadin and glutenin proteins from 1BL/1RS translocation lines with quality Traits[D]. Shandong Agriculture University.)
郝晨阳. 2001. 甘肃春小麦种质资源贮藏蛋白遗传变异研究[D]. 甘肃农业大学, (Hao Ch-Y. 2001. Study on genetic variation of storage protein in Gansu-grown spring wheat germplasm resources[D]. Gansu Agricultural University.)
马小乐. 2011. 甘肃小麦种质及其影响因素的分析[D]. 甘肃农业大学. (Ma X-L. 2011. Analysis of quality factors of spring wheat varieties in Gansu Province[D]. Gansu Agricultural University).
唐建卫, 刘建军, 张平平, 等. 2008. 小麦Glu-1位点变异和1B/1R易位对谷蛋白亚基表达量和面包加工品质的影响. 作物学报, 34(4): 571-577. (Tang J-W, Liu J-J, Zhang P-P, et al. 2008. He Zh-H. Effect of Allelic Variation at the Glu-1 Loci and 1B/1R Translocation on the Quantity of Gluten Protein Fractions and Pan Bread Making Quality in Common Wheat. ACTA AGRONOMICA SINICA, 34(4): 571-577.)
唐建卫, 刘建军, 张平平, 等. 贮藏蛋白组分对小麦面团流变学特性和食品加工品质的影响[J].中国农业科学, 2008, 41(10): 2937-2946. (Tang J-w, Liu J-j, Zhang P-p, et al. 2008. Effects of Gluten Protein Fractions on Dough Property and Products Quality in Common Wheat. Scientia Agricultura Sinica, 2008, 41(10): 2937-2946)
余利, 何方, 陈桂玲, 等. 2011. 利用1RS特异标记和染色体原位杂交技术鉴定小麦1BL/1RS易位系[J]. 作物学报, 37(3): 563-569. (Yu L, He F, Chen G-L, et al. 2011. Identification of 1BL/1RS wheat-rye chromosome translocations via 1RS specific molecular markers and genomic in situ hybridization. [J]. ACTA AGRONOMICA SINICA, 37(3): 563-569.)
张玲丽, 李秀全, 杨欣明,等. 2006. 小麦优良种质资源高分子量麦谷蛋白亚基组成分析[J]. 中国农业科学, 39(12): 2406-2414. (Zhang L-l, Li X-q, Yang X-m, et al. 2006. High-Molecular-Weight Glutenin Subunit Composition of Chinese Wheat Germplasm. Scientia Agricultura Sinica, 39(12): 2406-2414)
张平平, 陈东升, 张勇, 等. 2006. 春播小麦醇溶蛋白组成及其对品质性状的影响[J]. 作物学报, 32: 1796-1801. (Zhang P-P, Chen D-S, Zhang Y, et al. 2006. Gliadin Composition and their effects on quality properties in spring wheat[J]. ACTA AGRONOMICA SINICA, 32: 1796-1801.)
