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2025年8月14日 星期四
  2015, Vol. 23 Issue (3): 311-319    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
水稻淀粉合成相关基因SSⅠ、SSⅢ-1和PUL对稻米品质的影响
康翠芳1,向珣朝2,龙小林2,苏文丽2,许顺菊2
1. 西南科技大学生命科学与工程学院
2. 西南科技大学
Effects of the Starch-synthesizing Genes SSⅠ, SSⅢ-1 and PUL on Rice(Oryza sativa L.) Quality
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摘要 可溶性淀粉合成酶基因(soluble starch synthaseⅠ gene, SSⅠ)和SSⅢ-1以及极限糊精酶基因(pullulanase, PUL)是淀粉合成过程中参与支链淀粉合成的关键基因。为了研究SSⅠ、SSⅢ-1和PUL对稻米(Oryza sativa L.)品质的影响,本研究以颗粒结合淀粉合成酶基因(granule bound starch synthas, Wx)与SSⅡ-3均相同的籼型(indica)光温敏核不育系广占63S与潜力三系恢复系CG173R为亲本构建的B1C1F9株系作为供试材料,分析回交重组自交系(backcross inbred lines, BILs)各株系的蒸煮食味品质。结果表明,双亲仅在焦磷酸化酶基因大亚基基因(ADP-glucose pyrophos-phorylase large subunit ADPG, AGPlar)、分支酶3基因(starch branching enzymeⅢ gene, SBE3)、PUL、SSⅠ和SSⅢ-1基因位点存在差异;SSⅠ、SSⅢ-1和PUL基因在B1C1F9株系中分离对蒸煮品质指标具有显著影响。SSⅢ-1基因在后代分离过程中,不同基因型之间碱消值(alkaline spreading value, ASV)、峰值粘度(peak viscosity, PKV)、崩解值(breakdown value, BDV)和峰值时间(peak time, PeT)4个指标具有显著性差异;PUL基因在后代分离过程中,不同基因型之间,胶稠度(gel consistency, GC)和回复值(consistence value, CSV)具有显著性差异(P<0.05);在SSⅢ-1和SSⅠ基因同时分离的株系中,裂区试验分析表明,SSⅢ-1和SSⅠ基因的互作效应对糊化温度(gelatinization temperature, GT)的效应达极显著水平(P<0.01),对表观直链淀粉含量(apparent amylose content, AAC)、GC、PKV和ASV的效应达显著水平(P<0.05)。说明SSⅢ-1和PUL基因对稻米蒸煮食味品质的部分理化指标有显著影响,且SSⅢ-1和SSⅠ基因之间存在显著的互作效应。在相同Wx和SSⅡ-3基因基因背景下,研究稻米淀粉合成途径效应较小的基因对稻米食味品质的影响,对改良稻米蒸煮食味品质和加快稻米品质育种研究具有重要意义。
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康翠芳
向珣朝
龙小林
苏文丽
许顺菊
关键词 水稻蒸煮食味品质可溶性淀粉合成酶基因(SSⅠ)SSⅢ-1基因脱分支酶基因(PUL)回交重组自交系(BILs)    
Abstract:Soluble starch synthaseⅠ(SSⅠ), SSⅢ-1 and pullulanase (PUL) genes are involved in the synthesis process of amylopectin. In order to study their effects on rice (Oryza sativa L.) quality, we used backcross inbred lines (BILs) as materials which were constructed by indica photo-thermo-sensitive gene male sterile (PTGMS) line Guangzhan 63S and rice potential restorer line CG173R which contained the same alleles of granule bound starch synthase gene (Wx) and SSⅡ-3. Furthermore, the eating and cooking qualities were measured among the BILs. The results showed that there were 5 different starch synthesis-related genes(ADP-glucose pyrophos-phorylase large subunit ADPG (AGPlar), starch branching enzymeⅢ(SBE3), PUL, SSⅠand SSⅢ-1) for two parents; SSⅠ, SSⅢ-1 and PUL genes had significant effects on eating and cooking qualities in the B1C1F9 line among them. The 4 indices of physicochemical (alkali spreading value (ASV), peak viscosity (PKV), breakdown value (BDV) and peak time (PeT)) of the materials for different genotypes reached significantly different level(P<0.05) in the SSⅢ-1 gene separation population. On the PUL gene loci, the significant difference existed on the gel consistency (GC) and consistence value(CSV) for different genotypes. Split plot analysis showed that interaction effects of SSⅢ-1 and SSⅠgene on gelatinization temperature(GT) reached extremely significant level(P<0.01), and the effects on apparent amylase content (AAC), GC, PKV and ASV reached significantly different level(P<0.05). Hence, it could be concluded that SSⅢ-1 and PUL genes had significant effects on the eating and cooking qualities and there was an apparent interaction effect between SSⅢ-1 and SSⅠgenes. In the same background of Wx and SSⅡ genes, the study of effects of minor genes among the starch synthesis-related genes on rice quality has importance for improving the rice eating and cooking qualities and accelerating the research on rice quality breeding.
Key wordsRice    Eating and cooking qualities    Soluble starch synthaseⅠgene (SSⅠ)    SSⅢ-1 gene    Pullulanase gene (PUL)    Backcross inbred lines (BILs)
收稿日期: 2014-09-04      出版日期: 2015-02-02
基金资助:四川省教育厅重点项目;四川省生物质资源利用与改性工程技术研究中心开放基金项目;西南科技大学研究生创新基金资助项目
通讯作者: 向珣朝     E-mail: xiangxunchao@swust.edu.cn
引用本文:   
康翠芳 向珣朝 龙小林 苏文丽 许顺菊. 水稻淀粉合成相关基因SSⅠ、SSⅢ-1和PUL对稻米品质的影响[J]. , 2015, 23(3): 311-319.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2015/V23/I3/311
 
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