Abstract：Hair follicle is a appendages of skin, and its periodic regeneration involves the activation and regulation of hair follicle stem cells. Cashmere goats (Capra hircus) skin tissue samples of different month age, was fixed, dehydrated, embeded, paraffined and sliced, which was finally stained by hematoxylin and eosin stain(HE). Three antibodies of keratin 15(K15), tyrosine-related protein 2(Trp2) and antibody of proliferating cell nuclear antigen 3(PCNA3) were used to determine the niche of hair follicle stem cells by immunohistochemistry. The results showed that Trp2 expressed in bulge of hair follicle in 2 kinds of hair follicle, but the signal was stronger in primery hair follicles; K15 mainly expressed outer root sheath under of bulge in telogen and expressed from under bulge to half of both hair folllicles; PCNA strongly expressed in anagen hair follicle but expressed weaker in telogen. Trp2 provides the site of the stem cell niche include diffirent stem cell types in hair follicles, K15 illustrates the migration trajectory of stem cell, PCNA reflects the propagation of hair follicle stem cells. All of the findings supply the foundation of finding the derect marker of hair follicles and regulation mechanism of hair follicle stem cells during the cashmere wool regeneration in Cashmere goats.
