Abstract：To investigate the effects of various physical and chemical factors on the growth and the hyoscyamine production in hairy root cultures of Anisodus acutangulus, the influence of different minimal mediums, carbon sources, growth regulators, pH, temperature and photoperiod were studied by liquid culture. The results showed that B5 medium was most suitable to the growth of hairy roots and the formation of hyoscyamine in hairy roots. Hairy roots growth and hyoscyamine formation were promoted when B5 was used as the basic medium, at pH 6.5, temperature of 25 ℃ and photoperiod of 18 h/d. The effect of 5% sucrose was better to hairy roots growth within carbon source concentrations of 1% to 7%, but hyoscyamine contents in hairy roots were decreased with the increase of carbon source concentration. 0.05 mg/L of NAA, IBA and 2,4-D all could promote the growth of hairy roots, but such growth regulator substances inhibited strongly scopolamine formation in hairy roots regardless of the concentration of high or low. This research on the effects of these physical and chemical factors can provide a scientific reference for the optimization of the culture conditions of Anisodus acutangulus hairy roots and the mass production of hyoscyamine.
