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影响体外受精生产奶牛性控胚胎的因素
赵学明1,杜卫华1,郝海生1,任晶晶2,王栋1,刘岩2,秦彤1,朱化彬1
1. 中国农业科学院北京畜牧兽医研究所
2.
Influence Factors on Producing Bovine Sexed Embryos by In vitro Fertilization with Sex-sorted Sperm
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摘要 为了促进性控精液体外受精胚胎的产业化应用,本实验从卵母细胞体外成熟后卵丘细胞脱除程度(不脱除~完全脱除)、种公牛个体(A~C)、性控精液受精滴体积(30~100 μL)和性控精子密度(0.3×106~1.0×106个/mL) 4个方面,研究影响牛性控精液体外受精效率的因素。结果表明:(1)卵丘细胞部分脱除组的卵裂率(67±4.74)%显著高于不脱除组(41±2.23)%和完全脱除组(37.50±2.88)% (P<0.05);(2)种公牛B的性控精液进行体外受精,卵裂率(61.25±5.78)%和囊胚率(26.53±3.31)%显著高于A(42.5±3.45,17.65±3.65)%和C(30±2.62,16.67±2.36)% (P<0.05);(3)50~100 μL受精滴卵裂率(60±3.54)%~(64.17±3.04)%和囊胚率(20.41±4.33)%~(23.38±4.47)%无显著性差异(P>0.05),但显著高于40、30 μL的卵裂率[(48.33±2.89)%, (33±2.74)%]和囊胚率[(13.79±2.02)%, (12.12±3.73)%] (P<0.05); (4)精子密度0.5×106~1.0×106个/mL的卵裂率(62±4.12)%~(68.75±4.64)%和囊胚率(22.58±2.17)%~(27.27±3.49)%相比无显著差异(P>0.05),但显著高于0.3×106个/mL组卵裂率(38.75±3.58)%和囊胚率(16.13 ±3.46)%(P<0.05)。结果提示,采用适宜的种公牛精液、卵丘细胞部分脱除,结合较小的受精滴体积、较低的精子浓度,对于提高性控精液体外受精效率、降低性控胚胎体外生产成本具有重要意义。
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赵学明
杜卫华
郝海生
任晶晶
王栋
刘岩
秦彤
朱化彬
关键词 牛,性控精液,体外受精,卵母细胞    
Abstract:In order to enhance the industrial application of embryos produced by in vitro fertilization(IVF) with sex-sorted sperm, several main factors were investigated in the present study, including cumulus cell removal degree (intact, partial removal and complete removal), different bulls (A~C), volume of IVF drops (30~100 μL) and sperm concentrations(0.3×106~1.0×106/mL). The results showed that (1)The cleavage rate of oocytes with partial removal of cumulus cells (67±4.74)% was significantly higher than that of oocytes with intact cumulus cells (41±2.23)% or complete removal of cumulus cells (37.50±2.88)% (P<0.05); (2)The cleavage and blastocyst rates of oocytes fertilized with sex-sorted sperm of bull B [(61.25±5.78)%, (26.53±3.31)%] were significantly higher than those of bull A [(42.5±3.45)%, (17.65±3.65)%] and C [(30±2.62)%, (16.67±2.36)%] (P<0.05); (3)The cleavage and blastocysts rates of oocytes fertilized in 50~100 μL drops had no significant difference(60±3.54)%~(64.17±3.04)% and (20.41±4.33)%~(23.38±4.47)% (P>0.05), but significantly higher than those in 40 μL[(48.33±2.89)%, (13.79±2.02)%] or 30 μL [(33±2.74)%, (12.12±3.73)%](P<0.05);(4)The cleavage and blastocyst rates of oocytes fertilized with a sperm concentration of 0.5×106~1.0×106/mL showed no significant difference(62±4.12)%~(68.75±4.64)% and (22.58±2.17)%~(27.27±3.49)% (P>0.05), but were significantly higher than those with 0.3×106/mL[(38.75±3.58)%, (16.13±3.46)%] group(P<0.05). The results indicate that partial removal of cumulus cells and the selection of appropriate bulls combined with reasonable decrease of volume of drops and sperm concentration contribute greatly to increasing the efficiency of IVF with sex-sorted sperm, meanwhile decreasing the production cost of sexed embryos.
Key wordsBovine;Sex-sorted sperm;In vitro fertilization;Oocytes
收稿日期: 2011-01-24     
通讯作者: 朱化彬   
引用本文:   
赵学明1,杜卫华1,郝海生1,任晶晶2,王栋1,刘岩2,秦彤1,朱化彬1. 影响体外受精生产奶牛性控胚胎的因素[J]. , 2011, 19(5): 843-848.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2011/V19/I5/843
 
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