Abstract：Abstract Selection of the best stable reference genes is of crucial importance for the accurate normalization of expression in real-time quantitative PCR. Taking the piglet tissues as example, the expressions of nine housekeeping geens beta-actin(ACTB), beta-2-microglobulin(B2M), glyceraldehyde-3-phosphate dehydrogenase(GAPDH), hydroxymethylbilane synthase(HMBS), hypoxanthine phosphoribosyltransferase 1(HPRT1), ribosomal protein L4(RPL4), succinate dehydrogenase complex, subunit A(SDHA), TATA box binding protein1(TBP1) and Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein and zeta polypeptide(YWHAZ) were detected using real-time quantitative PCR. And their expression stability were observed by analysis of geNorm program. Results showed HPRT1 and HMBS > RPL4 > TBP1> B2M > YWHAZ > SDHA > GAPDH > ACTB. As the best choice, HMBS can be used as normalizing expression of target gene in different piglet tissues, while RPL4 is good reference gene for high abundant transcripts.
收稿日期: 2009-02-26
通讯作者:
台玉磊
引用本文:
台玉磊1,韩立强2,杨国庆2,王艳玲2,常磊2,臧 猛2,武宇晓2,王 静2,张志强2,杨国宇2. 仔猪组织基因表达中实时定量PCR内参基因的选择[J]. , 2010, 18(4): 732-736.
2, 2, 2, 2, 2, 2, 2, 2, 2. Selection of the Reference Genes of Real-time Quantitative PCR in the Gene Expression of Piglet Tissues. , 2010, 18(4): 732-736.
