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| Clone and characteristics of a novel gene HbUEP from latex in Hevea brasiliensis |
| Yun YANG Zhi-Li ZHANG Kuan-Can LIU Wei-Guo LI |
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Abstract HbUEP（Hevea brasiliensis ubiquitin extension protein）gene , a latex expressed gene, has been cloned and sequenced from latex in H.brasiliensis, based on the analysis of differentially expressed cDNA subtractive library induced by ethphon, indicating that the UEP gene cDNA has 771bp nucleotides, composed of 226bp 3`UTR,77bp 5`UTR and a 468bp length open reading frame encoding 156 amino acid peptide. Southern blot analysis showed this gene is a kind of low-copy genes in H. brasiliensis genomic. UEP gene lowly expressed in latex from control and rubber trees 6h after application of ethphon, and strongly expressed in latex from rubber trees 12h after application of ethphon within 24h after application of ethphon, showing that this gene expression can be regulated by ethphon. Ethphon can increase the latex yield and induce the senescence of latecifer in H.brasiliensis. It is suggested that UEP gene may be involved in the regulation of ethphon-induced high latex yield or senescence of latecifer in H.brasiliensis.
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Received: 17 July 2007
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Corresponding Authors:
Zhi-Li ZHANG
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