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Abstract For development of the Ee chromosome specific molecular markers for diploid species of L.elongatum, amplified fragment length polymorphism (AFLP) analysis was performed on common wheat Chinese Spring (CS), CS—L. elongatum disomic substitution and additional lines using 5 primer combinations, and 28 AFLP markers, which were specific for 1Ee to 7Ee chromosome of the diploid species of L.elongatum, respectively, were obtained. After cloning and sequencing of these AFLP fragments, four AFLP markers of E-ATC/M-CGTA341bp, E-ATT/M-CTAG265bp, E-AAT/M-CCAG139bp and E-ATT/M-CTAG205bp were successfully converted into reliable sequence tagged markers (STS). These four markers were further subject to amplification other five accessions of the L.elongatum and other eight common wheats without L.elongatum sources for validating their specificity and polymorphism. The results showed that these markers could amplification the same size of the fragment from L.elongatum accessions but couldn’t amplification it from other eight common wheat, suggesting that these markers could be used for identify and tracing the Ee chromosomes in wheat- L.elongatum substitution and additional lines.
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Received: 08 November 2007
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Corresponding Authors:
Ze-Hong Yan
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