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| Genome-wide Identification of LYK Gene Family in Arachis hypogaea and Expression Analysis in Response to Abiotic Stress |
| SHI Jun-Jie, ZHAO Ru, LI Xiao-Li, JI Jun-Bin, CAI Yao, ZHANG Wei, ZHU Chang-Qing, WANG Li-Ke, BEN Ai-Ling, LIU Ting-Li, BAO Ya-Zhou* |
| College of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China |
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Abstract Peanut (Arachis hypogaea) is an important oilseed and cash crop that is frequently affected by abiotic stresses such as drought and low temperature during production. To clarify the member composition, structural characteristics, and expression divergence of the lysin motif receptor-like kinase (LYK) gene family in allotetraploid cultivated peanut, genome-wide identification of LYK members was performed using the reference genome of the peanut cultivar 'Fuhuasheng'. Phylogenetic relationships, chromosomal locations, gene structures, conserved motifs, and promoter cis-acting regulatory elements were subsequently analyzed. Publicly available transcriptome data were further used to investigate tissue-specific expression and expression responses to phytohormones, drought, and low temperature, and the low-temperature responses of selected candidate genes were validated by RT-qPCR. The research results showed that a total of 20 LYK genes were identified and classified into 3 subgroups (Ⅰ, Ⅱ and Ⅲ) according to their phylogenetic relationships. These genes were unevenly distributed across the peanut chromosomes, and approximately half were intronless, suggesting their potential for rapid responses. Various light-responsive, hormone-responsive and stress-responsive cis-acting elements were widely distributed in their promoter regions, particularly those associated with abscisic acid and methyl jasmonate responses. Analysis of public transcriptome data showed that several LYK genes exhibited tissue-specific expression patterns. LYK8 was highly expressed in roots and root-related tissues, whereas LYK10 was expressed in roots, root-stem composite tissues, stems, and cotyledons, suggesting their possible involvement in root development or growth regulation in different tissues. Different LYK members exhibited distinct expression responses to phytohormones, drought, and low temperature. In particular, LYK2 showed a marked response to drought, whereas LYK3 was upregulated under multiple phytohormone and low-temperature conditions, indicating possible functional divergence among LYK members in hormone signaling and abiotic stress responses. Overall, the cultivated peanut LYK gene family shows marked divergence in gene structure, regulatory element composition, and expression patterns, and some members may participate in tissue development and abiotic stress responses. This study provides a reference for subsequent functional validation of peanut LYK genes and the identification of candidate genes for stress resistance.
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Received: 15 December 2025
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| Fund:国家自然科学基金(32673179; 32302309); 江苏省自然科学基金(BK20230986) |
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Corresponding Authors:
*Yazhou_Bao@163.com
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