解淀粉芽胞杆菌菌株YX3的分离鉴定及其对大豆根腐病的防控效果

doi:10.3969/j.issn.1674-7968.2026.08.012

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Abstract Soybean root rot is one of the major soil-borne diseases that severely impacts the production of soybean(Glycine max). Current control methods predominantly rely on chemical pesticides, which are susceptible to causing environmental pollution and promoting pathogen resistance. This situation underscores the urgent need for eco-friendly alternatives, such as microbial biocontrol agents. To screen for effective biocontrol bacteria against soybean root rot, the YX3 strain with antibacterial activity was obtained from the rhizosphere soil of maize(Zea mays). It was identified as Bacillus amyloliquefaciens by comprehensive analysis of colony morphology observation, physiological and biochemical characteristics, and muti-gene phylogenetic analysis. The dual-culture assay showed that YX3 had an inhibition rate of 68.98% on the mycelial growth of Fusarium oxysporum, one of the pathogens of soybean root rot, which could cause mycelial deformities of F. oxysporum, such as swelling, shriveled and twisted mycelium. The sterile fermentation filtrate of YX3 significantly inhibited the germination of F. oxysporum spores; Volatile metabolites interfered with the pigment metabolism of F. oxysporum, resulting in hypha fading, loose structure and weakened pathogenicity. Enzyme activity test showed that YX3 could secrete amylase, protease and cellulase, and its genome contained fenA, srfAA and ituD antibiotic genes. The crude lipopeptide extract had antibacterial activity against F. oxysporum. In greenhouse pot experiment, the control effect of YX3 on soybean root rot was 62.79%. In addition, the YX3 strain showed broad-spectrum antibacterial activity against a variety of plant pathogenic fungi(the antibacterial rate was 64.44%~88.74%). The research showed that YX3 has the potential to be developed as a biocontrol agent, which provides a new candidate resource and theoretical basis for biological control of soybean root rot.

Key words： Soybean root rot Fusarium oxysporum Bacillus amyloliquefaciens Biological control

Received: 09 September 2025

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S432.1

Corresponding Authors: ^*liuchunlai@163.com

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	DU Hong-Rui
	YANG Fan
	WANG Shuang
	JIANG Xi-Feng
	JIA Bei
	LIU Chun-Lai

Cite this article:

DU Hong-Rui,YANG Fan,WANG Shuang, et al. Isolation, Identification and Control Effects of Bacillus amyloliquefaciens YX3 Against Soybean(Glycine max) Root Rot[J]. 农业生物技术学报, 2026, 34(8): 1738-1749.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2026.08.012 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2026/V34/I8/1738

[1] 邓云, 田大刚, 苏妍, 等. 2023. 多粘类芽孢杆菌NPDY05-8对玉米茎基腐病的防治效果及对土壤微生物的影响[J]. 福建农业学报, 38(12): 1445-1452.
(Deng Y, Tian D G, Su Y, et al.2023. Control on maize stalk rot and effects on soil microbes of Paenibacillus polymyxa[J]. Fujian Journal of Agricultural Sciences, 38(12):1445-1452.)
[2] 东秀珠, 蔡妙英. 2021. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, pp. 189-255.
(Dong X Z & Cai M Y. 2021. Handbook of Identification of Common Bacterial Systems[M]. BeiJing: Science Press, pp. 189-255.)
[3] 马家新. 2018. 大豆根腐病拮抗内生细菌的筛选鉴定及其生防效果[D]. 硕士学位论文, 南京农业大学, 导师: 郑小波, pp. 16-17.
(Ma J X.2018. Isolation of soybean endophytic bacteria as candidate biological control agents for soybean root rot disease control[D]. Thesis for M. S., Nanjing Agricultural University, Supervisor: Zheng X B, pp. 16-17.)
[4] 钱新, 陈亚丽, 李美霞, 等. 2025. 辣椒疫霉病生防菌的筛选、防效及抑菌机理[J]. 植物病理学报, 55(05): 1-17.
(Qian X, Chen Y L, Li M X, et al.2025. Screening, control effect and antifungal mechanism of a bacterial biocontrol strain against pepper Phytophthora blight[J]. Acta Phytopathologica Sinica, 55(05): 1-17.)
[5] 石彬, 赵子璇, 谢学文, 等. 2025. 豇豆根腐病拮抗菌株ZF520的鉴定及其生防效果[J].农业生物技术学报, 33(10):2233-2243.
(Shi B, Zhao Z X, Xie X W, et al.2025. Identification of antagonistic strain ZF520 against cowpea(Vigna unguiculata) root rot disease and it's biocontrol effect[J]. Journal of Agricultural Biotechnology, 33(10):2233-2243.)
[6] 王娇, 刘晓宁, 焦甜甜, 等. 2025. 拮抗白术疫病病原菌的木霉菌的分离鉴定及其防效[J]. 农业生物技术学报, 33(04): 857-866.
(Wang J, Liu X N, Jiao T T, et al.2025. Isolation and identification of Trichoderma spp. antagonizing the pathogen of Atractylodes macrocephala blight disease and their control effect[J]. Journal of Agricultural Biotechnology, 33(04): 857-866.)
[7] 王爽, 李新民, 刘春来, 等. 2023. 引起黑龙江省大豆根腐病的镰刀菌种类鉴定及致病分析[J]. 植物病理学报, 53(01): 126-128.
(Wang S, Li X M, Liu C L, et al.2023. Identification and pathogenicity of Fusarium species causing soybean root rot in Heilongjiang Province[J]. Acta Phytopathologica Sinica, 53(01): 126-128.)
[8] 许子怡, 宋小飞, 赵文义, 等. 2025. 解淀粉芽孢杆菌JDF630脂肽抑制菌活性与成分鉴定[J]. 微生物学通报, 52(06): 2630-2638.
(Xu Z Y, Song X F, Zhao W Y, et al.2025. Antifungal activity and components of lipopeptides from Bacillus amyloliquefaciens JDF630[J]. Microbiology China, 52(06): 2630-2638.)
[9] 张学雯, 张学彬, 李红亚, 等. 2019. 黄曲霉拮抗菌Bacillus amyloliquefaciens B10-6-1产生的脂肽类抗生素的分离和鉴别[J]. 菌物学报, 38(04): 594-602.
(Zhang X W, Zhang X B, Li H Y, et al.2019. Isolation and identification of lipopeptide antibiotic produced by Bacillus amyloliquefaciens B10-6-1 antagonistic to Aspergillus flavus[J]. Mycosystema, 38(04): 594-602.)
[10] 朱文静. 2011. 大豆生物种衣剂的研制与应用[D]. 硕士学位论文, 南京农业大学, 导师: 高学文, pp. 31.
(Zhu W J. 2011. Development and application of biological seed coating formulation for soybean[D]. Thesis for M. S., Nanjing Agricultural University, Supervisor: Gao X W, pp. 31.)
[11] Ahmed W, Zhou G S, Yang J, et al.2022. Bacillus amyloliquefaciens WS-10 as a potential plant growth-promoter and biocontrol agent for bacterial wilt disease of flue-cured tobacco[J]. Egyptian Journal of Biological Pest Control, 32: 25.
[12] Buchanan R, Bergey N.1994. Bergey's Manual of Determinative Bacteriology[M]. 9th Edition. Baltimore: Williams and Wilkins Company, pp. 205-315.
[13] Chun J, Bae K S.2000. Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences[J]. Antonie van Leeuwenhoek, 78(2): 123-127.
[14] Fan Z, Feng J, Zheng L, et al.2025. Bacillus subtilis B579 controls cucumber Fusarium wilt by improving rhizosphere microbial community[J]. Microorganisms, 13(6): 1382.
[15] Hwang S H, Noh J S, Maung C E H, et al.2025. Sustainable management of southern blight in peanut using volatile organic compounds produced by Bacillus amyloliquefaciens HK17[J]. Microbial Pathogenesis, 206: 107829.
[16] Jiang N, Qiu J, Tian D, et al.2025. Mixture of Bacillus amyloliquefaciens and Bacillus pumilus modulates community structures of rice rhizosphere soil to suppress rice seedling blight[J]. Rice Science, 32(1): 118-130.
[17] Kim P I, Chung K C.2004. Production of an antifungal protein for control of Colletotrichum lagenarium by Bacillus amyloliquefaciens MET0908[J]. FEMS Microbiology Letters, 234(1): 177-183.
[18] Koumoutsi A, Chen X H, Henne A, et al.2004. Structural and functional characterization of gene clusters directing nonribosomal synthesis of bioactive cyclic lipopeptides in Bacillus amyloliquefaciens strain FZB42[J]. Journal of Bacteriology, 186(4): 1084-1096.
[19] Kumar H, Guleria S, Kuča K, et al.2025. Emerging prospects of Bacillus species in attaining the sustainable development goals[J]. Applied Microbiology and Biotechnology, 109(1): 242.
[20] Lalucat J, Mulet M, Gomila M, et al.2020. Genomics in bacterial taxonomy: Impact on the genus Pseudomonas[J]. Genes, 11(2):139.
[21] Li X J, Yao C X, Qiu R, et al.2023. Isolation, identification, and evaluation of the biocontrol potential of a Bacillus velezensis strain against tobacco root rot caused by Fusarium oxysporum[J]. Journal of Applied Microbiology, 134(1): lxac049.
[22] Sun L, Wang W, Zhang X, et al.2023. Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporum[J]. Frontiers in Microbiology, 14: 1275986.
[23] Tamura K, Stecher G, Kumar S.2021. MEGA11: Molecular evolutionary genetics analysis version 11[J]. Molecular Biology and Evolution, 38(7): 3022-3027.
[24] Vianna G R, Cunha N B, Rech E L.2023. Soybean seed protein storage vacuoles for expression of recombinant molecules[J]. Current Opinion in Plant Biology, 71: 102331.
[25] Wang C J, Wang Y Z, Chu Z H, et al.2020. Endophytic Bacillus amyloliquefaciens YTB1407 elicits resistance against two fungal pathogens in sweet potato(Ipomoea batatas (L.) Lam.)[J]. Journal of Plant Physiology, 253: 153260.
[26] Wei D, Zhu D, Zhang Y, et al.2024. Pseudomonas chlororaphis IRHB3 assemblies beneficial microbes and activates JA-mediated resistance to promote nutrient utilization and inhibit pathogen attack[J]. Frontiers in Microbiology, 15: 1328863.
[27] Xi X D, Fan J L, Yang X Y, et al.2022. Evaluation of the anti-oomycete bioactivity of rhizosphere soil-borne isolates and the biocontrol of soybean root rot caused by Phytophthora sojae[J]. Biological Control, 166: 104818.
[28] Yang P, Yuan P, Liu W, et al.2024. Plant growth promotion and plant disease suppression induced by Bacillus amyloliquefaciens strain GD4a[J]. Plants(Basel, Switzerland), 13(5): 672.
[29] Yi Y, Luan P, Fan M, et al.2024. Antifungal efficacy of Bacillus amyloliquefaciens ZK-9 against Fusarium graminearum and analysis of the potential mechanism of its lipopeptides[J]. International Journal of Food Microbiology, 422: 110821.
[30] Zalila-Kolsi I, Ben-Mahmoud A, Al-Barazie R.2023. Bacillus amyloliquefaciens: Harnessing its potential for industrial, medical, and agricultural applications-a comprehensive review[J]. Microorganisms, 11(9): 2215.
[31] Zhang N, Wang Z, Shao J, et al.2023. Biocontrol mechanisms of Bacillus: Improving the efficiency of green agriculture[J]. Microbial Biotechnology, 16(12): 2250-2263.