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| Screening of Single-copy Gene Conserved Sequence and Its Application in Germplasm Identification of Cephalopholis sonnerati |
| LIU Yang1,2, LU Sheng1,2, LI Ming1,2, WANG Chong-Wei1,2, ZHOU Bo3, CHEN You-Ming4, SONG Yu1,2, WANG Zi-Yuan1,2, CHEN Song-Lin1,2,* |
1 State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2 Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao 266237, China;
3 Wanning Linlan Aquaculture Co., Ltd., Wanning 571528, China;
4 Hainan Lanliang Technology Co., Ltd., Sanya 572025, China |
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Abstract The tomato hind, Cephalopholis sonnerati, is a new species of high economic value grouper, which is widely distributed, rich genetic diversity, and difficult to identify its germplasm. In order to develop molecular markers and methods suitable for germplasm identification of the tomato hind, this study used the single copy gene database of spinal animals combined with the resequencing data of the tomato hind to screen the highly conserved sequences of the tomato hind. A target sequence located in the single-copy gene ceramide glucosyltransferase(ugcg) was successfully selected, and specific primers were designed. A specific band of 226 bp with only individual base differences could be amplified from the tomato hind, but the corresponding band cannot be amplified from other groupers. In this study, a single copy conserved gene was selected as the core tool to establish a rapid and accurate identification method for the germplasm resources collection, identification and evaluation and also laid a foundation for the molecular breeding technology innovation and the artificial breeding of the tomato hind.
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Received: 25 September 2025
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Corresponding Authors:
*chensl@ysfri.ac.cn
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