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| Identification of NCED Gene in Hibiscus esculentus and Its Expression Analysis in Pods |
| LI Yong-Ping1,*, ZHENG Duo-Le2,*, ZHANG Hui1, QIU Bo-Yin1, XUE Zhu-Zheng1, ZHU Hai-Sheng1, WEN Qing-Fang1,** |
1 Crops Research Institute/Fujian Key Laboratory of Vegetable Genetics and Breeding, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
2 Faculty of Forestry, University of British Columbia, Vancouver V6T1Z2, Canada |
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Abstract Abscisic acid (ABA) serves as a pivotal hormone in plants, regulating the entire process of plant growth, development, and stress responses. Nine-cis-epoxycarotenoid dioxygenase (NCED) acts as the rate-limiting enzyme in ABA biosynthesis, directly determining its biosynthetic efficiency and content. The identification and analysis of the NCED gene in Hibiscus esculentus are crucial for elucidating the mechanism of ABA-mediated regulation of pod senescence. In this study, the HeNCED gene in H. esculentus was identified and analyzed using bioinformatics. The results revealed that the full length of HeNCED gene (GenBank No. PZ350883) was 2 196 bp, with an open reading frame (ORF) of 1 803 bp encoding 600 amino acids. The protein was localized in the cytoplasm, exhibiting acidic, hydrophilic, and unstable properties. Sequence similarity analysis showed over 88% identity with homologous proteins from Gossypium anomalum, G. raimondii, G. arboreum, and H. syriacus, indicating high conservation. qPCR analysis of pod samples at different developmental stages and post-harvest treated with exogenous ABA and nordihydroguaiaretic acid (NDGA) demonstrated a significant positive correlation between HeNCED expression and ABA content (P<0.01). It was hypothesized that HeNCED regulated secondary wall thickening in H. esculentus pods via modulating ABA synthesis. This study preliminarily revealed the molecular mechanism of HeNCED in Hibiscus esculentus pod aging, which can provide a theoretical basis and genetic resources for molecular breeding, cultivation, and post-harvest storage of H esculentus.
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Received: 12 August 2025
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Corresponding Authors:
**fjvrc@163.com
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| About author:: *These authors contributed equally to this work |
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