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| Preparation and Photodynamic Antibacterial Effect Evaluation of Silver Metal-organic Framework Ag-BTC |
| WANG Xing-Wu1, LI Jiang-Bo2, PAN Wan-Bing1, ZHANG Di1, LIN Jia-Hao1,* |
1 College of Veterinary Medicine/ State Key Laboratory of Veterinary Public Health and Safety, China Agricultural University, Beijing 100193, China;
2 Jilin Province Liaoyuan City Xi'an District Agriculture, Forestry and Water Resources Work Station, Liaoyuan 136200, China |
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Abstract Metal-organic frameworks (MOFs) represent a class of emerging porous crystalline materials constructed from metal ions serving as nodes and organic ligands acting as linkers. They exhibit high porosity, extensive specific surface area, and excellent biocompatibility, rendering them suitable for diverse applications in biomedical fields, gas storage, trace element detection, and sensing. Through the strategic design of specific metal ion and organic ligand combinations, MOFs can generate efficient photodynamic effects, thereby garnering considerable research interest in antibacterial and antitumor applications. In this study, silver metal-organic framework (Ag-BTC) was synthesized via a solvothermal method employing silver nitrate (AgNO3) and 1,3,5-benzenetricarboxylic acid (BTC) as precursors. The physicochemical properties of Ag-BTC were characterized. Its light-induced reactive oxygen species (ROS) generation capacity and pH-responsive behavior were investigated using electron paramagnetic resonance (EPR) and inductively coupled plasma-optical emission spectrometry (ICP-OES). Antibacterial activity against Staphylococcus pseudintermedius was evaluated via the spread plate method and scanning electron microscopy (SEM). The results demonstrate that, under simulated sunlight irradiation for 10 minutes, Ag-BTC simultaneously produced singlet oxygen (1O2) and hydroxyl radicals (·OH). Notably, its decomposition efficiency in weakly acidic conditions was approximately two-fold higher compared to neutral environments, indicating pronounced pH-responsive characteristics. The antibacterial rate against S. pseudintermedius reached 97.4%, inducing bacterial membrane shrinkage, perforation, and DNA leakage, corroborating its significant antibacterial effects. This study provides a theoretical foundation for the potential application of Ag-BTC in the veterinary clinical treatment of bacterial skin infections.
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Received: 15 August 2025
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Corresponding Authors:
*jiahao_lin@cau.edu.cn
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[1] Jia A, Zheng M, Xie Z.2025. Boron dipyrromethene fungicide for anti-microbial photodynamic therapeutics[J]. Journel of Photochemistry and Photobiology B-Biolohy, 265:113137.
[2] Jiang Z C, Li J Y, Wang J H, et al.2024. Multifunctional fucoidan-loaded Zn-MOF-encapsulated microneedles for MRSA-infected wound healing[J]. Journel of Nanobiotechnology, 22(1):152.
[3] He J R, Feng Y, Jiang J R, et al.2023. Preparation and characterization of a sustained-release antibacterial melamine-impregnated paper based on Ag-BTC[J].Journal of Materials Science, 58:6727-6742.
[4] Hillier A, Lloyd D H, Weese J S, et al.2014. Guidelines for the diagnosis and antimicrobial therapy of canine superficial bacterial folliculitis (Antimicrobial Guidelines Working Group of the International Society for Companion Animal Infectious Diseases)[J]. Veterinary Dermatology, 25(3):163-e43
[5] Li L F, Gao X Y, Li M C, et al.2024. Relationship between biofilm formation and antibiotic resistance of Klebsiella pneumoniae and updates on antibiofilm therapeutic strategies[J]. Frontiers in Cellular and Infection Microbiology, 14:1324895.
[6] Li Z, Wu X, Wu S, et al.2022. An effective and economical encapsulation method for trapping lead leakage in rigid and flexible perovskite photovoltaics[J]. Nano Energy, 93:106853.
[7] Loeffler A, Cain C L, Ferrer L, et al.2025. Antimicrobial use guidelines for canine pyoderma by the International Society for Companion Animal Infectious Diseases (ISCAID)[J]. Veterinary Dermatology, 36:234-282.
[8] Lynch S A, Helbig K J.2021. The complex diseases of Staphylococcus pseudintermedius in canines:Where to next?[J]. Veterinary Sciences, 8(1):11.
[9] Mu R Z, Chen Y F, Chen D L, et al.2026. Metal-organic frameworks for natural medicines delivery:Stimuli-responsive strategies and synergistic cancer therapy applications[J]. Chemical Communications, 62(9):2841-2863.
[10] Rodríguez-Diéguez A.2022. Antibacterial Activity of Two Zn-MOFs Containing a Tricarboxylate Linker[J]. Nanomaterials, 12(23):4139.
[11] Sahu K, Krishna H, Shrivastava R, et al.2024. Evaluation of the potential of Delta-aminolevulinic acid for simultaneous detection of bioburden and anti-microbial photodynamic therapy of MRSA infected wounds in Swiss albino mice[J]. Journal of Photochemistry and Photobiology. B:Biology, 254:112892.
[12] Wang G, Chen H, Wu L, et al.2026. A novel ROS switcher potentiates the type-I photodynamic effect of sodium zinc chlorophyllin against Pseudomonas aeruginosa in diabetic wounds[J]. Biomaterials Advances, 182:214721.
[13] Yu W, Chen S, Zhu J, et al.2023. A highly dispersed and surface-active Ag-BTC catalyst with state-of-the-art selectivity in CO2 electroreduction towards CO[J]. Journal of CO2 Utilization, 70:102457. |
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