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| Identification and Analysis of HSP20 Gene Family in Artemisia argyi |
| ZANG Qiao-Lu*, ZHENG Xiao-Mei*, LIU Lu, WANG Meng, MIAO Ru-Yi** |
| College of Horticulture, Shanxi Agricultural University, Taigu 030801, China |
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Abstract Heat shock protein 20 (HSP20) is a class of small molecular proteins that play critical roles in responding to temperature and other abiotic stresses. Identification and analysis of AaHSP20 gene family members in Artemisia argyi is helpful to reveal the regulatory mechanism response to temperature. 24 AaHSP20 genes were identified by using bioinformatics, and their physicochemical properties, evolutionary relationships, expression patterns, and so on were analyzed. The results showed that the encoded proteins ranged from 155 to 215 amino acids (aa) in length, the molecular weight was 17.60~24.67 kD, and most were predicted to be located in the cytoplasm. The secondary structures of AaHSP20 proteins were mainly composed of α-helix and random coils. The 24 AaHSP20 genes were unevenly distributed across 10 chromosomes. Some AaHSP20 genes had collinear relationships with homologs in Arabidopsis thaliana and Lactuca sativa. Phylogenetic analysis showed that the 24 AaHSP20 proteins could be divided into 3 classes and 4 subfamilies. All AaHSP20 proteins contained conserved domains and motifs (motif 1 and motif 2), and most genes lacked introns. Protein interaction analysis suggested AaHSP20 might interact with other small heat shock proteins. qRT-PCR analysis demonstrated that 5 candidate AaHSP20 genes exhibited distinct expression patterns in different tissues. AaHSP20-2, AaHSP20-7, AaHSP20-16, and AaHSP20-21 were sensitive to both high and low temperatures, while AaHSP20-20 was only sensitive to high temperature. These findings provide a theoretical foundation for elucidating the functions of AaHSP20 genes and improving resistance breeding of A. argyi.
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Received: 02 December 2024
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Corresponding Authors:
**mry1964@126.com
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| About author:: *These authors contributed equally to this wor |
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