水稻<em>Waxy</em>基因第四内含子3'剪接位点邻近碱基突变影响水稻抗性淀粉含量

doi:10.3969/j.issn.1674-7968.2025.09.001

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Abstract In recent years, with the rising incidence of typeⅡ diabetes, resistant starch (RS) has gained significant attention as a low-glycemic index food component. Resistant starch offers important physiological benefits, such as reducing postprandial blood glucose levels in diabetic patients and decreasing the risk of intestinal dysfunction and colon cancer. The content of resistant starch in rice (Oryza sativa) is positively correlated with amylose content, and the synthesis of amylose is controlled by the Waxy (Wx) gene. In this study, CRISPR/Cas9 gene editing technology was employed to edit the bases near the 3' splice site AG of the fourth intron of the Wx gene in rice, resulting in 4 different types of mutant lines. By analyzing the expression levels of the Wx gene, the activity of granule-bound starch synthase (GBSS), and the contents of amylose and resistant starch in these mutant lines, it was found that mutations near the 3' splice site AG significantly affected the expression of the Wx gene, thereby regulating the contents of amylose and resistant starch in rice. Correlation analysis revealed a significant positive correlation between Wx gene expression and resistant starch content. This study identified intron mutation genotypes that can effectively regulate the expression level of the Wx gene (either increasing or decreasing it), providing new insights for future improvements in the resistant starch content of rice.

Key words： Rice Resistant starch Waxy Gene editing

Received: 17 February 2025

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Corresponding Authors: ^**jianfzhang@163.com

About author:: ^*These authors contributed equally to this work

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	LUO Xi
	FAN Jia-Xing
	WEI Yi-Dong
	WEI Lin-Yan
	ZHU Yong-Sheng
	HE Wei
	WU Fang-Xi
	CAI Qiu-Hua
	XIE Hua-An
	ZHANG Jian-Fu

Cite this article:

LUO Xi,FAN Jia-Xing,WEI Yi-Dong, et al. The Impact on Resistant-starch Content for Base Mutations Near the 3' Splice Site of the Fourth Intron of the Waxy Gene in Rice (Oryza sativa)[J]. 农业生物技术学报, 2025, 33(9): 1873-1882.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.09.001 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I9/1873

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