番茄红素对H<sub>2</sub>O<sub>2</sub>诱导的猪卵巢颗粒细胞氧化损伤的保护作用

doi:10.3969/j.issn.1674-7968.2025.07.010

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Abstract The ovaries of sows (Sus scrofa) suffer oxidative stress can affect their reproductive performances. Lycopene (LYC), a natural carotenoid, has strong antioxidant properties and effectively alleviate oxidative damage in the body.The aim of this work was to investigate the protective effect of LYC against hydrogen peroxide (H₂O₂)-induced apoptosis and oxidative damage in porcine ovarian granulosa cells (PGCs). In this study, healthy and sexually mature porcine ovarian tissues were collected to isolate and culture PGCs, dividing the experiment into 3 groups: The control group, the H₂O₂ model group, and the LYC+H₂O₂ treatment group. Cell counting kit-8 (CCK-8) was used to detect cell viability to screen the optimal concentration of H₂O₂ damage and LYC treatment, and the kits were used to detect ROS and related antioxidant indexes. qRT-PCR was used to detect the mRNA expression levels of the genes related to cell apoptosis B cell lymphoma-2 (BCL-2), BCL-2-associated X protein (BAX), cysteinyl aspartate-specific proteinase-3 (CASP3), cell proliferation proliferating cell nuclear antigen (PCNA), insulin-like growth factor 2 (IGF2), antioxidant enzyme system catalase (CAT), manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), and steroid hormone biosynthesis steroidogenic acute regulatory protein (STAR), 3β-hydroxysteroid dehydrogenase 1 (HSD3B1), cytochrome P450 11A1 (CYP11A1), cytochrome P450arom (CYP19A1). Results indicated that 5 μmol/L LYC was most effective in promoting PGC viability, while 200 μmol/L H₂O₂ represented the IC₅₀ for PGCs. Compared to the control group, the H₂O₂ model group showed extremely significantly elevated intracellular ROS fluorescence, ROS levels, and malondialdehyde (MDA) content (P<0.01), alongside decreased levels of total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px), and catalase (CAT) (P<0.05). The mRNA expression levels of BCL-2, PCNA, IGF2, CuZnSOD, MnSOD, CAT, STAR, HSD3B1, CYP11A1and CYP19A1 were extremely significantly downregulated in the H?O? model group (P<0.01), while BAX, CASP3 were extremely significantly upregulated (P<0.01) compared with the control group. In the LYC + H?O? group, intracellular ROS fluorescence, ROS levels, and MDA content extremely significantly decreased compared to the H?O? model group (P<0.01), whereas cell viability, T-SOD, T-AOC, GSH-Px, and CAT levels extremely significantly increased (P<0.01). Additionally, LYC treatment significantly upregulated the mRNA expression of PCNA, IGF2, BCL-2, CuZnSOD, MnSOD, CAT, STAR, CYP11A1, and CYP19A1 (P<0.05) and significantly downregulated BAX and CASP3 (P<0.05) compared with the H₂O₂ model group. These results suggested that LYC had a protective effect on porcine ovarian granulosa cells by alleviating oxidative stress damage caused by H₂O₂. This study provides theoretical basis for the application of LYC in the swine industry.

Key words： Lycopene (LYC) Oxidative stress Porcine ovarian granulosa cells (PGCs) Apoptosis Reproductive performances

Received: 21 October 2024

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Corresponding Authors: ^*zheng780911@163.com

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	YANG Zhen-Qing
	HU Guang-Ling
	SHI Qing-Wu
	LI Chang-Hong
	CHEN Guang
	WANG Jie
	ZHANG Yi-Yu
	AO Zheng

Cite this article:

YANG Zhen-Qing,HU Guang-Ling,SHI Qing-Wu, et al. Protective Effect of Lycopene Against H₂O₂-induced Oxidative Damage in Porcine Ovarian Granulosa Cells[J]. 农业生物技术学报, 2025, 33(7): 1531-1542.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.07.010 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I7/1531

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